Scripps Research Institute
Han Chen
500 University Drive, P.O.Box 850
Room C1730
Hershey, PA 17033
https://research.med.psu.edu/core-facilities/light-microscopy/
RRID: RRID:SCR_022526
Leica SP8 FCS/ FLIM
Light Sheet Fluorescent Microscope (LSFM)
Leica SP8 STED 3X Inverted Confocal
Nikon A1 MP+ Upright Multiphoton
DeltaVisionElite Deconvolution Inverted
Image processing workstation
Confocal Microscope With FCS/FLIM Capabilities
Confocal Microscopy
Confocal/ Super Resolution Microscope
Microscopy
Multiphoton Microscopy
Super-resolution Microscopy
1.) Lambert GS, Siedlecki CA, Parent LJ (2025 Jun 13). Two Peas in a Pod: Retroviral RNA Dimers Organize Gag–RNA Nanoclusters with Novel Biophysical Properties International Journal of Molecular Sciences, 26(12), 5679. . PMCID: 12193034.
2.) Lambert GS, Siedlecki CA, Parent LJ (2025 Jun 11). Two peas in a pod: retroviral RNA dimers organize Gag-RNA nanoclusters with novel biophysical properties bioRxiv, (), 2025.06.09.658653. . PMCID: 12259046.
3.) Shaikh K, Bowman M, McCormick SM, Gao L, Zhang J, White J, Tawil J, Kapoor A, Arav-Boger R, Norbury CC, Harhaj EW (2025 Jan 17). ZFAND6 promotes TRAF2-dependent mitophagy to restrain cGAS-STING signaling. iScience, 28(1), 111544. . PMID: 39811672.
4.) Lambert GS, Maldonado RJ, Parent LJ (2025 Jan 13). Role of the Psi Packaging Signal and Dimerization Initiation Sequence in the Organization of Rous Sarcoma Virus Gag-gRNA Co-Condensates Viruses, 17(1), 97. . PMCID: 11769450.
5.) Maldonado RJK, Parent LJ (2025 Jan 11). Dynamic interactions of retroviral Gag condensates with nascent viral RNA at transcriptional burst sites: implications for genomic RNA packaging. bioRxiv : the preprint server for biology, (), . . PMID: 39829876.
6.) White J, Choi YB, Zhang J, Vo MT, He C, Shaikh K, Harhaj EW (2025 Jan). Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregates. Autophagy, 21(1), 160-177. . PMID: 39193925.
7.) Hamamoto K, Liang X, Ito A, Lanza M, Bui V, Zhang J, Opozda DM, Hattori T, Chen L, Haddock D, Imamura F, Wang HG, Takahashi Y (2024 Dec 24). Unveiling the physiological impact of ESCRT-dependent autophagosome closure by targeting the VPS37A ubiquitin E2 variant-like domain. Cell reports, 43(12), 115016. . PMID: 39607828.
8.) Tang M, Dirks K, Kim SY, Qiu Z, Gao Y, Sun D, Peruggia G, Sallavanti J, Li W (2024 Dec 23). Inhibition of thioredoxin reductase 1 sensitizes glucose-starved glioblastoma cells to disulfidptosis. Cell death and differentiation, (), . doi: 10.1038/s41418-024-01440-0. PMID: 39715824.
9.) Bui V, Liang X, Ye Y, Giang W, Tian F, Takahashi Y, Wang HG (2024 Dec 18). Blocking autophagosome closure manifests the roles of mammalian Atg8-family proteins in phagophore formation and expansion during nutrient starvation. Autophagy, (), . doi: 10.1080/15548627.2024.2443300. PMID: 39694607.
10.) Straka J, Khatib JB, Pale L, Nicolae CM, Moldovan GL (2024 Nov 18). CAF-1 promotes efficient PrimPol recruitment to nascent DNA for single-stranded DNA gap formation Nucleic Acids Research, 52(22), 13865-13880. . PMCID: 11662685.
11.) Hanafiah A, Geng Z, Liu T, Tai YT, Cai W, Wang Q, Christensen N, Liu Y, Yue F, Gao Z (2024 Nov 15). PRC1 and CTCF-Mediated Transition from Poised to Active Chromatin Loops Drives Bivalent Gene Activation. bioRxiv : the preprint server for biology, (), . . PMID: 39605346.
12.) Pale LM, Khatib JB, Nusawardhana A, Straka J, Nicolae CM, Moldovan GL (2024 Nov 12). CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single-stranded DNA gaps Nucleic Acids Research, 52(22), 13832-13848. . PMCID: 11662931.
13.) Khatib JB, Dhoonmoon A, Moldovan GL, Nicolae CM (2024 Jul 23). PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis Nature Communications, 15(), 6197. . PMCID: 11266678.
14.) Khursheed A, Xu LC, Siedlecki CA (2024 Jul). The Effects of Submicron Textured Surface Topography on Antibiotic Efficacy against Biofilms Journal of biomedical materials research. Part B, Applied biomaterials, 112(7), e35436. . PMCID: 11239140.
15.) Torres MC, Rebok A, Sun D, Spratt TE (2024 Jul 1). Activity of DNA polymerase κ across the genome in human fibroblasts Proceedings of the National Academy of Sciences of the United States of America, 121(28), e2403130121. . PMCID: 11252913.
16.) Lu T, Yee PP, Chih SY, Tang M, Chen H, Aregawi DG, Glantz MJ, Zacharia BE, Wang HG, Li W (2024 Jul). LC3-associated phagocytosis of neutrophils triggers tumor ferroptotic cell death in glioblastoma. The EMBO journal, 43(13), 2582-2605. . PMID: 38806658.
17.) Khursheed A, Xu LC, Siedlecki CA (2024 Jul). The effects of submicron-textured surface topography on antibiotic efficacy against biofilms. Journal of biomedical materials research. Part B, Applied biomaterials, 112(7), e35436. . PMID: 38961592.
18.) Pale LM, Khatib JB, Nicolae CM, Moldovan GL (2024 Apr 20). CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps bioRxiv, (), 2024.04.17.589988. . PMCID: 11042333.
19.) Ye Y, Liang X, Wang G, Bewley MC, Hamamoto K, Liu X, Flanagan JM, Wang HG, Takahashi Y, Tian F (2024 Mar 15). Identification of membrane curvature sensing motifs essential for VPS37A phagophore recruitment and autophagosome closure Communications Biology, 7(), 334. . PMCID: 10942982.
20.) Lambert GS, Rice BL, Kaddis Maldonado RJ, Chang J, Parent LJ (2024 Jan 18). Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome bioRxiv, (), 2024.01.18.575255. . PMCID: 10827203.
21.) Kazzaz SA, Shaikh KA, White J, Zhou Q, Powell WH, Harhaj EW (2024 Jan). Phosphorylation of aryl hydrocarbon receptor interacting protein by TBK1 negatively regulates IRF7 and the type I interferon response. The Journal of biological chemistry, 300(1), 105525. . PMID: 38043800.
22.) Saha K, Subramenium Ganapathy A, Wang A, Arumugam P, Michael Morris N, Harris L, Yochum G, Koltun W, Perdew GH, Nighot M, Ma T, Nighot P (2023 Dec). Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation. Mucosal immunology, 16(6), 826-842. . PMID: 37716509.
23.) Chang J, Parent LJ (2023 Nov 22). HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery Journal of Virology, 97(12), e01179-23. . PMCID: 10734548.
24.) Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL (2023 Oct 7). Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases. Nature communications, 14(1), 6265. . PMID: 37805499.
25.) Hattori T, Fundora KA, Hamamoto K, Opozda DM, Liang X, Liu X, Zhang J, Uzun Y, Takahashi Y, Wang HG (2023 Sep 21). ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis Autophagy, 20(2), 349-364. . PMCID: 10813646.
26.) Pandya Shesh B, Slagle-Webb B, Shenoy G, Khristov V, Zacharia BE, Connor JR (2023 Sep). Uptake of H-ferritin by Glioblastoma stem cells and its impact on their invasion capacity. Journal of cancer research and clinical oncology, 149(12), 9691-9703. doi: 10.1007/s00432-023-04864-2. PMID: 37237166.
27.) Kaddis Maldonado R, Lambert GS, Rice BL, Sudol M, Flanagan JM, Parent LJ (2023 Aug 15). The Rous sarcoma virus Gag Polyprotein Forms Biomolecular Condensates Driven by Intrinsically-disordered Regions. Journal of molecular biology, 435(16), 168182. . PMID: 37328094.
28.) Maldonado RK, Rice BL, Lambert GS, Sudol M, Flanagan JM, Parent LJ (2023 Apr 8). The Rous sarcoma virus Gag polyprotein forms biomolecular condensates driven by intrinsically-disordered regions. bioRxiv : the preprint server for biology, (), . . PMID: 37066255.
29.) Yoshioka NK, Young GM, Khajuria DK, Karuppagounder V, Pinamont WJ, Fanburg-Smith JC, Abraham T, Elbarbary RA, Kamal F (2022 Jun 1). Structural changes in the collagen network of joint tissues in late stages of murine OA. Scientific reports, 12(1), 9159. . PMID: 35650306.
30.) Wei Y, Luo H, Yee PP, Zhang L, Liu Z, Zheng H, Zhang L, Anderson B, Tang M, Huang S, Li W (2021 Dec). Paraspeckle Protein NONO Promotes TAZ Phase Separation in the Nucleus to Drive the Oncogenic Transcriptional Program. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(24), e2102653. . PMID: 34716691.
31.) Hattori T, Takahashi Y, Chen L, Tang Z, Wills CA, Liang X, Wang HG (2021 Feb). Targeting the ESCRT-III component CHMP2A for noncanonical Caspase-8 activation on autophagosomal membranes. Cell death and differentiation, 28(2), 657-670. . PMID: 32807832.
32.) Grillo SL, Etzel JD, Weber SR, Ondeck C, Wang W, Zhao Y, Barber AJ, Sundstrom JM (2021 Feb). Descriptive analysis of Fibulin-3 and the extracellular vesicle marker, Alix, in drusen from a small cohort of postmortem human eyes. Experimental eye research, 203(), 108422. doi: 10.1016/j.exer.2020.108422. PMID: 33387484.
The Light Microscopy Imaging Core at Penn State College of Medicine provides consultation and training in ultra-high-resolution imaging of cells and tissues in fixed or live states. The core also provides expertise in quantitative image analysis and consultations on microscopy-related research projects.
