Dartmouth Cancer Center
Fred Kolling
1 Medical Center Dr
Rubin 670
LEBANON, NH 03756
https://cancer.dartmouth.edu/scientists-researchers/molecular-biology-resource
RRID: RRID:SCR_021293
Other Citation Identifiers P30CA023108-41
P30CA023108
10X Genomics Chromium Plus Genetic Analyzer
Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]
RRID:SCR_019326
Citation IDs: P20GM130454 S10OD025235
Illumina iScan System
Can scan thousands of array samples per day, without sacrificing data quality or reproducibility. [Product Link]
RRID:SCR_020128
Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
RRID:SCR_020140
Citation IDs: 1S10OD030242
Illumina NovaSeq 6000 Sequencing System
Unleashes groundbreaking innovations that leverage our technology Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. [Product Link]
RRID:SCR_020150
10x Genomics CytAssist
Citation IDs: P20GM130454
Vizgen MERSCOPE
Citation IDs: P20GM130454
ABI 3730 Genetic Analyzer
NanoString nCounter MAX system
10x Genomics
ATAC-Seq
DNA Analysis
DNA Isolation
Genomics
Genotyping
Metagenomics Analysis
Methylation Analysis
Microarray
Nanopore Sequencing
Nanostring
Nanostring NCounter
Ribosomal Reduction
RNA analysis
RNA Integrity
RNA Isolation
Sequencing - DNA Sequencing
Sequencing - Next Generation Sequencing (NGS)
Single-Cell Analysis
Single-cell Sequencing
Single-molecule Imaging
Spatial Transcriptomics
Whole Exome Sequencing
Whole Genome Sequencing
1.) Potts CM, Yang X, Lynes MD, Malka K, Liaw L (2025 Mar 17). Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 14(6), e038465. . PMCID: 12132619.
2.) Saxe R, Stuart H, Marshall A, Abdullahi F, Chen Z, Emiliani F, McKenna A (2025 Mar 3). Hierarchical Lineage Tracing Reveals Diverse Pathways of AML Treatment Resistance bioRxiv, (), 2025.02.27.640600. . PMCID: 11908168.
3.) Sajani A, Schaafsma E, Croteau W, ElTanbouly M, Nowak EC, Cheng C, Burns CM, Turk MJ, Noelle RJ, Lines JL (2025 Jan 10). Heterogeneity and plasticity of the naïve CD4+ T cell compartment Research Square, (), rs.3.rs-5744327. . PMCID: 11760246.
4.) Huynh TN, Havrda MC, Zanazzi GJ, Chang CCY, Chang TY (2024 Oct 14). Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Myelin Debris-Treated Microglial Cell Lines Activates the Gene Expression of Cholesterol Efflux Transporter ABCA1. Biomolecules, 14(10), . . PMID: 39456234.
5.) Tau S, Chamberlin MD, Yang H, Marotti JD, Roberts AM, Carmichael MM, Cressey L, Dragnev C, Demidenko E, Hampsch RA, Soucy SM, Kolling F, Samkoe KS, Alvarez JV, Kettenbach AN, Miller TW (2024 Sep 27). Endocrine persistence in ER+ breast cancer is accompanied by metabolic vulnerability in oxidative phosphorylation. bioRxiv : the preprint server for biology, (), . . PMID: 39386444.
6.) Rosner GM, Goswami HB, Sessions K, Mendyka LK, Kerin B, Vlasac I, Mellinger D, Gwilt L, Hampton TH, Graber M, Ashare A, Harris WT, Christensen B, Stanton BA, Swiatecka-Urban A, Skopelja-Gardner S (2024 Sep 19). Lung-Kidney Axis in Cystic Fibrosis: Early Urinary Markers of Kidney Injury Correlate with Neutrophil Activation and Worse Lung Function medRxiv, (), 2023.11.10.23298378. . PMCID: 11451629.
7.) Gadgil P, Ballew O, Sullivan TJ, Lacefield S (2024 Sep 3). Aneuploidy of Specific Chromosomes is Beneficial to Cells Lacking Spindle Checkpoint Protein Bub3. bioRxiv : the preprint server for biology, (), . . PMID: 39282354.
8.) Lee MK, Azizgolshani N, Shapiro JA, Nguyen LN, Kolling FW, Zanazzi GJ, Frost HR, Christensen BC (2024 Apr 30). Identifying tumor type and cell type-specific gene expression alterations in pediatric central nervous system tumors. Nature communications, 15(1), 3634. . PMID: 38688897.
9.) Bagheri M, Lee MK, Muller KE, Miller TW, Pattabiraman DR, Christensen BC (2024 Mar). Alteration of DNA methyltransferases by eribulin elicits broad DNA methylation changes with potential therapeutic implications for triple-negative breast cancer. Epigenomics, 16(5), 293-308. . PMID: 38356412.
10.) Romo BA, Karakyriakou B, Cressey L, Brauer BL, Yang H, Warren A, Johnson AL, Kettenbach AN, Miller TW (2024 Feb 20). TRIM33 Is a Co-Regulator of Estrogen Receptor Alpha. Cancers, 16(5), . . PMID: 38473207.
11.) Wang Z, Lindgren KM, Loros JJ, Dunlap JC (2024). The auxotrophic formate (for) mutant of Neurospora crassa has significantly delayed growth but a normal circadian clock Fungal genetics reports, 68(), 1. . PMCID: 11565437.
12.) Sudo K, Delmas-Eliason A, Soucy S, Barrack KE, Liu J, Balasubramanian A, Shu CJ, James MJ, Hegner CL, Dionne HD, Rodriguez-Palacios A, Krause HM, O'Toole GA, Karpen SJ, Dawson PA, Schultz D, Sundrud MS (2024). Quantifying Forms and Functions of Enterohepatic Bile Acid Pools in Mice. Cellular and molecular gastroenterology and hepatology, 18(6), 101392. . PMID: 39179177.
13.) Yeum D, Renier TJ, Carlson DD, Ballarino GA, Lansigan RK, Meyer ML, Loos RJF, Emond JA, Masterson TD, Gilbert-Diamond D (2024). Genetic associations with neural reward responsivity to food cues in children. Frontiers in nutrition, 11(), 1387514. . PMID: 39385774.
14.) James MR, Aufiero MA, Vesely EM, Dhingra S, Liu KW, Hohl TM, Cramer RA (2023 Oct 12). Aspergillus fumigatus cytochrome c impacts conidial survival during sterilizing immunity mSphere, 8(6), e00305-23. . PMCID: 10871163.
15.) Traphagen NA, Schwartz GN, Tau S, Roberts AM, Jiang A, Hosford SR, Marotti JD, Goen AE, Romo BA, Johnson AL, Duffy EK, Demidenko E, Heverly P, Mosesson Y, Soucy SM, Kolling F, Miller TW (2023 Sep 15). Estrogen Therapy Induces Receptor-Dependent DNA Damage Enhanced by PARP Inhibition in ER+ Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 29(18), 3717-3728. . PMID: 37439680.
16.) Schwartz GN, Kaufman PA, Giridhar KV, Marotti JD, Chamberlin MD, Arrick BA, Makari-Judson G, Goetz MP, Soucy SM, Kolling F, Demidenko E, Miller TW (2023 Aug 1). Alternating 17β-Estradiol and Aromatase Inhibitor Therapies Is Efficacious in Postmenopausal Women with Advanced Endocrine-Resistant ER+ Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 29(15), 2767-2773. . PMID: 37260292.
17.) Rawat K, Tewari A, Li X, Mara AB, King WT, Gibbings SL, Nnam CF, Kolling FW, Lambrecht BN, Jakubzick CV (2023 Jun 5). CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes. The Journal of experimental medicine, 220(6), . . ID: 36946983.
18.) Levy JJ, Zavras JP, Veziroglu EM, Nasir-Moin M, Kolling FW, Christensen BC, Salas LA, Barney RE, Palisoul SM, Ren B, Liu X, Kerr DA, Pointer KB, Tsongalis GJ, Vaickus LJ (2023 Jun). Identification of Spatial Proteomic Signatures of Colon Tumor Metastasis: A Digital Spatial Profiling Approach. The American journal of pathology, 193(6), 778-795. doi: 10.1016/j.ajpath.2023.02.020. ID: 37037284.
19.) Bagheri M, Mohamed GA, Ashick M, Saleem M, Ognjenovic NB, Lu H, Kolling FW, Wilkins OM, Das S, Croix IS, Nagaraj SH, Muller KE, Gerber SA, Miller TW, Pattabiraman DR (2023 Apr 21). Pharmacological Induction of mesenchymal-epithelial transition chemosensitizes breast cancer cells and prevents metastatic progression. bioRxiv : the preprint server for biology, (), . . ID: 37131809.
20.) Fatemi M, Feng E, Sharma C, Azher Z, Goel T, Ramwala O, Palisoul SM, Barney RE, Perreard L, Kolling FW, Salas LA, Christensen BC, Tsongalis GJ, Vaickus LJ, Levy JJ (2023). Inferring spatial transcriptomics markers from whole slide images to characterize metastasis-related spatial heterogeneity of colorectal tumors: A pilot study. Journal of pathology informatics, 14(), 100308. . ID: 37114077.
21.) Li X, Kolling FW, Aridgides D, Mellinger D, Ashare A, Jakubzick CV (2022 Nov). ScRNA-seq expression of IFI27 and APOC2 identifies four alveolar macrophage superclusters in healthy BALF. Life science alliance, 5(11), . . ID: 35820705.
22.) Rawat K, Soucy SM, Kolling FW, Diaz KM, King WT, Tewari A, Jakubzick CV (2022 Oct 1). Natural Antibodies Alert the Adaptive Immune System of the Presence of Transformed Cells in Early Tumorigenesis. Journal of immunology (Baltimore, Md. : 1950), 209(7), 1252-1259. . ID: 36028292.
23.) Downey-Kopyscinski SL, Srinivasa S, Kisselev AF (2022 Jul 27). A clinically relevant pulse treatment generates a bortezomib-resistant myeloma cell line that lacks proteasome mutations and is sensitive to Bcl-2 inhibitor venetoclax Scientific Reports, 12(), 12788. . PMCID: 9329464.
24.) Divakar P, Reeves J, Gong J, Kolling FW 4th, Jack Hoopes P, Wegst UGK (2022 Jan 15). High-plex expression profiling reveals that implants drive spatiotemporal protein production and innate immune activation for tissue repair. Acta biomaterialia, 138(), 342-350. doi: 10.1016/j.actbio.2021.10.018. ID: 34673228.
25.) Molodtsov AK, Khatwani N, Vella JL, Lewis KA, Zhao Y, Han J, Sullivan DE, Searles TG, Preiss NK, Shabaneh TB, Zhang P, Hawkes AR, Malik BT, Kolling FW 4th, Usherwood EJ, Wong SL, Phillips JD, Shirai K, Angeles CV, Yan S, Curiel TJ, Huang YH, Cheng C, Turk MJ (2021 Sep 14). Resident memory CD8(+) T cells in regional lymph nodes mediate immunity to metastatic melanoma. Immunity, 54(9), 2117-2132.e7. . ID: 34525340.
26.) Koeppen K, Nymon A, Barnaby R, Bashor L, Li Z, Hampton TH, Liefeld AE, Kolling FW, LaCroix IS, Gerber SA, Hogan DA, Kasetty S, Nadell CD, Stanton BA (2021 Jul 13). Let-7b-5p in vesicles secreted by human airway cells reduces biofilm formation and increases antibiotic sensitivity of P. aeruginosa. Proceedings of the National Academy of Sciences of the United States of America, 118(28), . . ID: 34260396.
27.) Han J, Zhao Y, Shirai K, Molodtsov A, Kolling FW, Fisher JL, Zhang P, Yan S, Searles TG, Bader JM, Gui J, Cheng C, Ernstoff MS, Turk MJ, Angeles CV (2021 Mar). Resident and circulating memory T cells persist for years in melanoma patients with durable responses to immunotherapy. Nature cancer, 2(3), 300-311. . ID: 34179824.
28.) Podnar JW, Pantano L, Zeller MJ, Kolling FW, Zhang Y, Alekseyev YO, Niece J, Deiderick H, Fan J, Xuei X, Kieleczawa J, Levine SS, Herbert ZT, Adams M (2020 Sep). Cross-Site Evaluation of Commercial Sanger Sequencing Chemistries. Journal of biomolecular techniques : JBT, 31(3), 88-93. . ID: 32831655.
29.) Chamberlin MD, Wells JD, Shee K, Bean JR, Marotti JD, Wells WA, Trask HW, Kolling FW, Bhatt A, Kaufman PA, Schwartz GN, Gemery JM, McNulty NJ, Tsapakos MJ, Barth RJ, Arrick BA, Gui J, Miller TW (2020 Aug). Plasma DNA as a "liquid biopsy" incompletely complements tumor biopsy for identification of mutations in a case series of four patients with oligometastatic breast cancer. Breast cancer research and treatment, 182(3), 665-677. . ID: 32562118.
30.) Herbert ZT, Thimmapuram J, Xie S, Kershner JP, Kolling FW, Ringelberg CS, LeClerc A, Alekseyev YO, Fan J, Podnar JW, Stevenson HS, Sommerville G, Gupta S, Berkeley M, Koeman J, Perera A, Scott AR, Grenier JK, Malik J, Ashton JM, Pivarski KL, Wang X, Kuffel G, Mesa TE, Smith AT, Shen J, Takata Y, Volkert TL, Love JA, Zhang Y, Wang J, Xuei X, Adams M, Levine SS (2020 Jul). Multisite Evaluation of Next-Generation Methods for Small RNA Quantification. Journal of biomolecular techniques : JBT, 31(2), 47-56. . ID: 31966025.
31.) Todd KL, Baker KL, Eastman MB, Kolling FW, Trausch AG, Nelson CE, Conover JC (2017 Mar 22). EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche. The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(12), 3331-3341. . ID: 28258169.
32.) Mickelsen LE, Kolling FW 4th, Chimileski BR, Fujita A, Norris C, Chen K, Nelson CE, Jackson AC (2017 Sep-Oct). Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis. eNeuro, 4(5), . . ID: 28966976.
Services and instrumentation that enable DNA/RNA extraction and quality control, next-generation sequencing, epigenetic profiling and microarray analysis on a whole-genome scale, from the level organisms to single cells. Sanger Sequencing and Gene Expression assays with the NanoString nCounter Max System. We care for a wide range of molecular biology related instruments available for use by individuals on a walk-up basis.
