The Harvard Center for Biological Imaging was opened May 17, 2010 and is one of the most innovative biological imaging centers in the world. In order to ensure investigators always have access to the latest and best technologies, the HCBI was designed to be an “Evergreen” microscopy facility. Imaging systems at the HCBI are leased rather than purchased and replaced every 2-3 years. This ensures that the facility remains relevant and is never in possession of outdated equipment. Currently, the HCBI contains 20 microscopes and 12 high-end processing stations including confocal, super-resolution, multi-photon, lightsheet, live cell imaging, high-content screening, slide scanning, spatial transcriptomics, laser dissection and macroscopy. The facility also features wet lab space, eukaryotic and prokaryotic incubation, and access to nearby animal care facilities for a wide range of species.

We analyse all types of high-throughput sequencing data from RNA-seq and single cell RNA-seq to variant sequencing (exome or whole genome), to ChIP-seq or bisulfite sequencing. With spatial profiling methods becoming more accessible, we have developed new workflows for spatial transcriptomic analysis and multimodal integration. We offer a dedicated bioinformatics training program for researchers interested in learning basic data skills and the analysis of high-throughput sequencing data. Our workshops focus on utilizing best practices in the field and research reproducibility.

Core provides in vitro HIV-1 neutralizing antibody assay services. Serves as resource for assessing breadth and potency of HIV-1 neutralizing antibodies, and for characterizing neutralization phenotype of HIV-1 isolates. Investigators send monoclonal antibodies or serum samples from vaccinated or HIV-1-infected individuals to the core for in vitro testing using the validated TZM.bl neutralizing antibody assay. The core also has the capability to phenotype primary HIV-1 isolates for sensitivity to broadly neutralizing antibodies. GCLP-compliant, In vitro testing of monoclonal antibodies or seurm samples, Validated TZM.bl neutralizing antibody assay, Phenotoyping of primary HIV-1 isolates.

Our goal is to advance research efforts in the life sciences that cannot readily be accomplished in the traditional academic laboratory because of a need for expensive instrumentation or automation, scientific or organizational infrastructure, or multidisciplinary expertise.

To promote cutting-edge research and to foster scientific collaborations, we make our extensive resources available to scientists at Harvard. Our technical staff provide expertise and hands-on training and the use of instrumentation for a nominal fee. Researchers can sign up to use the instrumentation through an on-line scheduling system and conduct their experiments independently.

Proteomics and Mass Spectrometry Services, Quantitative Proteomics, LCMSMS, HPLC, C-terminal analysis, SILAC, FASP, MudPIT, GeLC, TMT quantitation and PTM analysis.