Preclinical I&T’s mission is to assist the biomedical research community in evaluating disease and therapy within a complete biological system in-vivo. To do so, we focus on the use of minimally invasive and humane tools, with a strong focus in visualization and computation. Pharmacokinetic, pharmacodynamic, toxicity and dosing estimations can be performed with a variety of Preclinical I&T’s multi-modal services and technologies such as:

Digital imaging study design
Execution of pilot studies and preclinical trials
Optical Imaging: Fluorescence and Bioluminescence
Positron Emission Tomography (PET)
Radiotheranostics (Radioligand Therapy)
Quantitative gamma counting
Micro Computerized X-ray Tomography (microCT)
Ultrasound and Photoacoustic Imaging (USPA)
Magnetic Resonance Imaging (MRI)
Cryo-Fluorescence Tomography (CFT)
Image analysis workstations
Specialized data and imaging analysis workflows and software tool development

For more details please refer to the facility’s website: https://preclinical-imaging.mit.edu and https://ki-sbc.mit.edu/preclinical-imaging-testing

The BioMicro Center is an integrated genomics/bioinformatics facility located at MIT. The core supports sample isolation, library preparation, short and long read sequencing, single cell and spatial analysis, bioinformatics, and data management.

We provide services and instrumentation for imaging and characterization of bio- and nanomaterials with a strong focus on biological sample preparation. Some instruments are available for independent use. We also provide full service TEM, cryoTEM, SEM/FIB and cryoSEM/FIB, cryoFluorscence, sample preparation and imaging. Additionally, we conduct CLEM and cryoCLEM workflows utilizing cryoFluorescence, cryoSEM and cryoFIB with a focus on bio samples. In some cases the endpoint imaging is in the SEM/FIB (2D or 3D volumeEM). In other instances samples are transferred to one of a selection of cryoTEMs. We also provide immunoEM. In addition to imaging services we provide training on instruments for independent user access to: SEM, TEM, rheometer, AFM, DLS, and Spectradyne (counting & sizing nanoparticles). Our instruments and services are available to both non-profit and for-profit entities.

Core offers automation and screening capabilities. Equipped with environmentally-controlled high-content microscopes, automated liquid handlers, plate washers and readers, and robotic platforms, HTS aims to accelerate experiments from small-scale assays to large screening campaigns.

The Histology Core at KI-MIT processes a large variety of large and small animal tissues for paraffin/frozen sectioning and IHC. We also work up a large variety of samples with drug delivery devices and soft tissue constructs.

This Core Facility provides MIT and Boston area researchers with technical expertise, training and access to sophisticated instrumentation, enabling and supporting the use of a wide range of flow cytometry techniques. This technology allows simultaneous multiparametric analysis of many thousands of cells per second, enabling trained researchers to rapidly analyze complex cell populations using benchtop analysis flow cytometers. High-speed assisted cell sorting services provide researchers with fast, objective and quantitative recording of fluorescent signals from individual cells combined with physical separation of cells of particular interest.

The Microengraving technology platform enables measuring multiple traits from individual immune cells, either in their native condition or upon stimulation. The modularity of the platform allows simultaneous assessment from small or large numbers of cells. Additionally, it is possible to obtain genetic information from the individual cells of interest.
To date, the platform has been employed in projects related to antibody discovery, tumor profiling from various cancer tissues, immune cell functions in various allergic, infectious or auto immune diseases.

This shared resource of the MIT Koch Institute provides fee-for-service support to investigators who utilize cultured stem cells such as organoids or novel mouse models to study human diseases.

We provide a customizable set of service options to match the specific needs of each project, including consultative advice and troubleshooting, complete tissue culture and microinjection servces within our dedicated facilities or hands-on training to enable investigators to perform these experiments either at their own laboratory or within our facilities.

The Barbara K. Ostrom (1978) Bioinformatics & Computing Facility is a shared institutional resource providing KI investigators with support, assistance and training in a wide range of bioinformatics topics. The core also maintains a high performance computing resource offering many bioinformatics applications, substantial processing power and secure storage and provides support for desktop computing and poster printing. Access is available to all members of the MIT community, to the extent permitted by available capacity. In recognition of funding support, priority access is given to members of the Core’s sponsoring entities (Koch Institute, MIT Biology, MIT Biological Engineering and MIT Center for Environmental Health Science) and NCI-funded research projects. In special circumstances, access may be available to non-MIT users (details available on request from the Facility Director, Charlie Whittaker)

Fee-or-Service lab providing the following: "Next-gen" DNA/RNA sequencing; Sanger sequencing; small scale and multiple peptide synthesis; larger scale peptide synthesis; peptide cellulose array synthesis; MALDI-TOF mass spectrometry and HPLC analytical and preparative scale.