The Core generates induced pluripotent stem cell (iPSC) lines from somatic cells including peripheral blood mononuclear cells and fibroblasts. The Core has established standard operating procedures for:
1) Pluripotent stem cell growth and maintenance
2) Genome editing technologies including CRISPR-CAS9
3) Differentiation to germ layer and derivative tissues of interest.
The Core provides enrichment training courses for investigators interested in learning how to work with pluripotent stem cells and/or establish the pluripotent stem cell technology in their own labs.

The Human Pluripotent Stem Cell Core, established in 2008 by the Raymond G. Perelman Center for Cellular and Molecular Therapeutics at Children’s Hospital of Philadelphia, serves the research needs of CHOP, the University of Pennsylvania, and outside academic communities in the field of human pluripotent stem cell biology.

The Gastrointestinal Epithelium Modeling (GEM) Core is a component of the GEM Program - a collaborative research effort focused on accelerating discovery and innovation using patient tissue-derived organoids. GEM Core staff generate and maintain a living biobank of tissue-derived organoids with matched original tissue and blood specimens and provide access to specialized training, protocols, and reagents.

The CHOP Biorepository Resource Center (BioRC) serves to accession, process, store, and manage distribution of research biospecimens for biobanking efforts. We offer specimen processing, and RNA and DNA extraction services.

Core has established quality controlled protocols to quantitatively analyze whole proteomes, phosphoproteomes, ubiquitylomes, and/or lysine acetylomes at deep level through process of serial enrichment.