The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry, and validated antibody panels for immune profiling on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours.

Core staff provide technical expertise, hardware and software training, operate the facility‘s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.

Core provides Cryo-EM services focused mostly on Single Particle Analysis. Housed microscopes include a Titan Krios with Gatan K3 detector.

We support internal and external clients with Cryo-EM services focused mostly on Single Particle Analysis. Our microscopes include a Titan Krios with Gatan K3 detector.

The SBP Viral Vector Core Facility provides state-of-the-art viral vector-based gene/shRNA delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, adeno-associated virus, zika virus, coronavirus, sindbis virus and vesicular stomatitis virus products as well as customized "The Works" viral vector construction and swapping service packages. There are over 50 ready-to-transduce reporter viruses available for live cell imaging and 2D/3D cell-based assays. For custom virus production, we can achieve high-titer, top quality viral particles suitable for in vitro and in vivo studies. The automated mini-scale preparations of vector plasmids and viruses in 96-well format can immediately allow downstream viral array screening. The core also extends technical expertise in non-viral nanoparticles which includes large extracellular vesicles and exosome purification, analysis and engineering.

The SBP Genomics Core provides end-to-end genomics solutions, from experimental design through sequencing, for academic and industry researchers. Our team offers full-service library preparation, high-accuracy sequencing on the Element Biosciences AVITI24, and access to the latest single-cell, spatial and epigenetic technologies.

Our services include:
- Next generation sequencing: RNA-seq, whole genome, whole exome and targeted sequencing
- Epigenetic profiling: DNA methylation, ATAC-seq, CUT&RUN, CUT&Tag and TIP-ChIP
- Single-cell multiomics: 10x Genomics Chromium X and Illumina PIP-seq
- Spatial transcriptomics and epigenomics: Curio Trekker, Illumina StrataMap, 10x Visium HD and GeoMx DSP
- In situ cell screening: Element Biosciences Cytos multiomic CRISPR and drug screening
- Sample QC, STR cell line authentication and instrument training

The SBP Functional Genomics Core Facility provides the infrastructure for cell-based gain-of-function (ORF, CRISPRa and CRISPRon) and loss-of-functioning (siRN, miRNA, shRNA, CRSIPRko, CRISPRi and CRISPRoff), libraries screening services, starting with assay development and carrying all the way through verification of identified targets. The large scale screens is accomplished via ready-to-use genome-wide or pathway-specific library platforms with choice of arrayed or pooled libraries. The FG core facility is equipped to perform custom CRISPR gene activation, knock-down, knock-out and editing services. We also adopt CRISPR nucleotide base editing technology and offer custom built CRISPR domain libraries. This approach can reveal the specific domain of a target protein interacting with another molecule, such as protein, IncRNA or drug in a native cell cellular environment.

The Sanford Burnham Prebys Proteomics Shared Resource provides state-of-the-art mass spectrometry-based proteomics services including identification, quantification and characterization of proteins and their post-translational modifications. We provide proteomics services to Institute scientists as well as outside non-profit and for-profits organization on a fee-for-service basis.

Academic drug discovery facility with advanced

The Sanford Burnham Prebys Cancer Metabolism Core provides a unique service for the analysis of metabolism, offering both metabolite quantification and stable-isotope tracing methodology, centered around gas chromatography-mass spectrometry (GC-MS). We offer GC-MS analysis of polar metabolites (TCA cycle, glycolysis metabolites and amino acids), short chain fatty acids, fatty acids and cholesterol, sugars and sugar-phosphates. As well as cells, we can also analyze tissues, tumors, serum or culture medium as necessary. We also have available a YSI 2950 analyzer for measurement of glucose, lactate and glutamine in medium samples. We collaborate closely with Core users and can provide in-depth advice on the direction of metabolic experiments, as well as the interpretation of results.