The Sanford Burnham Prebys Cancer Metabolism Core provides a unique service for the analysis of metabolism, offering both metabolite quantification and stable-isotope tracing methodology, centered around gas chromatography-mass spectrometry (GC-MS). We offer GC-MS analysis of polar metabolites (TCA cycle, glycolysis metabolites and amino acids), short chain fatty acids, fatty acids and cholesterol, sugars and sugar-phosphates. As well as cells, we can also analyze tissues, tumors, serum or culture medium as necessary. We also have available a YSI 2950 analyzer for measurement of glucose, lactate and glutamine in medium samples. We collaborate closely with Core users and can provide in-depth advice on the direction of metabolic experiments, as well as the interpretation of results.

The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours.

Core staff provide technical expertise, hardware and software training, operate the facility\'s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.