The Charité Experimental MRI laboratories at Charité Campus Mitte offer two 7 Tesla Bruker MRI machines for preclinical research. Users have access to a wide range of established methods in the field of neuroimaging of anatomy, tissue microstructure, structural/functional connectivity, but also in the field of functional and molecular MRI of tumors, kidney, placenta or heart.

ZeBanC is an integrated healthcare biobank that operates under certified conditions and strict SOPs. This enables biomedical and translational researchers to perform their analyses using high-quality biospecimens and data to generate reliable and reproducible research data. To meet the needs of our various stakeholders, we offer a modular biobanking portfolio that can cover the entire workflow from blood collection to storage, including all pre-analytical steps and their documentation. This includes access to existing biospecimens and data under well-defined and agreed conditions. Furthermore, we offer a spectrum of additional services ranging from DNA/RNA extraction and virtual microscopy to the entire management of sample and data flow for national/international research consortia. Last but not least, we provide secure conventional and automated storage of samples at various temperatures as well as secure data management.

Preclinical (mice, rats) Radionuclide Imaging with SPECT/CT and PET/MR, radiochemistry, tracer biokinetics and kinetic modeling

Transgenic Service providing
• Generation of transgenic mice via pronucleus injection (TALEN, CrispR Cas9) and ES-cell based techniques including ES-cell culture
• Cryopreservation of mice via Sperm and Embryos
• Rederivation
• De novo ES-cell establishment
• De novo establishment of Trophoblast cells

We are generating viral vectors for Charite members and external non-profit academic institutions.

The Core Facility offers thin sectioning, transmission electron microscopy (TEM), Cryo-TEM, confocal light microscopy and scanning electron microscopy (SEM). Advice is given routinely for adequate project programming. Projects are closely monitored during the course of evaluations by the core facility staff. The core facility is equipped with the necessary LM, TEM and SEM devices and a technical staff takes care of all the technical work. The director (Prof. Bachmann), vice director (Prof. Vida) and an additional technical assistant plus a photographer help to extend and control project work in the core facility.

The mission of the MPI-CBG Light Microscopy Facility (MPI-CBG LMF) is to provide 24/7 access to state-of-the-art light microscopy techniques and instruments as well as to follow future trends. We assist through consultation, training, and project collaborations.

The LMF advises users during design and execution of their imaging projects. We provide support with selecting the most suitable imaging techniques, microscope systems, and setting up imaging experiments.

We offer our expertise in imaging techniques, such as

Spinning disk confocal microscopy
Laser scanning confocal microscopy
2-photon microscopy
Light sheet microscopy
Wide-field microscopy
Total internal reflection fluorescence (TIRF) microscopy
Laser capture microdissection
Extended resolution microscopy
Quantitative phase imaging
Live cell and organoid imaging
Photomanipulation

Furthermore, the LMF is constantly exploring new commercial technologies that can support the research mission of the MPI-CBG. We are also involved in multiple outreach and educational activities in Dresden, Germany and beyond.