primary_duns:
161202122
1111 Highland Avenue
7016 WIMR
Madison, WI 53705
United Stateshttps://cancer.wisc.edu/research/resources/flow/cite this facility
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology, and treatment in basic, translational, and clinical research projects.
The Flow Lab was established in 1988 as a resource for the University of Wisconsin–Madison campus. The facility maintains two laboratories: the main facility located in the Wisconsin Institutes for Medical Research (WIMR) a full service satellite facility in the UW Biotechnology Center.
is_active:
Nobudget_end:
08/23/2020date_added:
08/25/2018agency_code:
NIHfiscal_year:
2018project_num:
1S10OD025225-01award_amount:
$600,173.00budget_start:
08/24/2018org_name:
UNIVERSITY OF WISCONSIN-MADISONorg_state:
WIorg_country:
UNITED STATESorg_zipcode:
537151218primary_duns:
161202122abstract_text:
Abstract The University of Wisconsin Comprehensive Cancer Center (UWCCC) Flow Cytometry Laboratory (Flow Lab) is a heavily utilized shared service on the University of Wisconsin-Madison campus available to researchers who are members as well as non-members of the UWCCC. The impact of the Flow Lab on biomedical research infrastructure at UW-Madison is significant. This High-End Instrumentation Grant is a request for funds that cover 77.5% (remaining 22.50% supported by the institution) of the total cost of a BD FACS Symphony™ instrument to further expand single cell analysis capabilities into the 30+ parameter range. Single cell analysis by flow cytometry is in the midst of an explosion of technologies and reagents to support such high parameter analyses. Current users are pushing the limits of existing technologies with panels in the 12-18 color range. The experienced staff, well-established daily Quality Control Procedures, Online Scheduling System, Educational Programs and the Computational and Statistical support available at the University of Wisconsin allow for seamless integration of the BD FACSymphony into the Flow Lab. The BD FACSymphony will impact the development of novel treatment strategies for various human pathologies and increase our understanding of human biology. The BD FACSymphony will support the research of seven major users- (i) clinical trials for development of a prostate cancer vaccine (PI, McNeel), (ii) development of anti-fungal and antimicrobial immunity (PI, Klein), (iii) understanding molecular mechanisms in leukemia and hematopoiesis (PI, Zhang), (iv) identifying environmental determinants of rhinovirus illness severity (PI, Gern), (v) developing rapid systems for isolating circulating tumor cells (PI, Lang), (vi) understanding transcriptional regulation of GATA switches (PI, Bresnick) and (vii) developing immunotherapeutic agents for melanoma (PI, Sondel). In addition, this cytometer will also support the funded research conducted by 12 minor users. The research focus of the major and minor users covers the gamut of major human maladies and integrates basic, translational and clinical research projects. All projects will benefit from the multi-parameter capabilities of the BD FACSymphony and will allow researchers to study rare cell populations and provide fundamental knowledge of disease processes. The BD FACSymphony requested through this proposal will enable analysis of 35 fluorescent markers and 2 scatter parameters. This same instrument can, in the future, be expanded to conduct analysis of up to 50 parameters. Therefore, this BD FACSymphony will help address the advanced flow cytometry needs of our users for several years in the future. The large major and minor user base ensures that the BD FACSymphony will be utilized for at least 75% of the total AUT. Outstanding continued support from the institution will ensure full financial solvency. A dedicated staff with over 32 years of collective experience in flow cytometry will help maintain a well- tuned BD FACSymphony and assist our users in conducting experiments that are rigorous and reproducible.project_title:
BD FACSymphony High Parameter Flow Cytometercontact_pi_name:
PATANKAR, MANISH Stotal_cost:
$600,173.00is_active:
Nobudget_end:
04/30/2015date_added:
05/03/2014agency_code:
NIHfiscal_year:
2014project_num:
1S10OD018202-01award_amount:
$350,505.00budget_start:
05/01/2014org_name:
UNIVERSITY OF WISCONSIN-MADISONorg_state:
WIorg_country:
UNITED STATESorg_zipcode:
537151218primary_duns:
161202122abstract_text:
DESCRIPTION (provided by applicant): The University of Wisconsin Carbone Cancer Center (UWCCC) Flow Cytometry Laboratory (Flow Lab) is a heavily utilized shared service on the University of Wisconsin-Madison campus available to researchers who are members as well as non-members of the UWCCC. The impact of the Flow Lab on biomedical research infrastructure at UW-Madison is significant. This Shared Instrumentation Grant is a request for funds that cover 80% (remaining 20% supported by the institution) of the total cost of a BD LSR Fortessa" (Fortessa) instrument to further enhance the capability and the capacity of the Flow Lab. The addition of the Fortessa will alleviate the 2-3 week wait times currently experienced by our users allowing timely analysis of clinical research samples. It will provide high laser power for increased resolution of cellular subpopulations and excitation wavelengths (561nm and 355nm) necessary for detection of newly developed fluorescent markers, and will include a High-Throughput Sampler option for increased efficiency and screening capability. This instrument will provide redundancy when the existing 5-laser LSR is undergoing maintenance and/or repair. The experienced staff, well-established daily Quality Control Procedures, Online Scheduling System and Educational Programs allow for seamless integration of the Fortessa into the Flow Lab. The Fortessa cytometer will impact the development of novel treatment strategies for various human pathologies and increase our understanding of human biology. Funded projects by major and minor users benefiting by addition of the Fortessa include (i) clinical trials for development of a prostate cancer vaccine (Doug McNeel), (ii) immunotherapeutic agents for melanoma (Paul Sondel), (iii) mouse models for CMML (Jing Zhang), (iv) rat models for genetic modulation of mammary cancer (Michael Gould), (v) modulating immune responses in ovarian cancer (Manish Patankar), (vi) Graft versus Host disease in allogeneic bone marrow transplant patients with hematologic malignancies, (vii) effect of biobehavioral factors on immune function (Erin Costanzo), (viii) fibrogenesis in kidney disease (Arjung Djamali), (ix) hematopoietic stem cell maturation into cardiomyocytes (Sean Palecek), (x) ventilatory control due to effects of inflammation on neuronal cells (Jyoti Watters), (xi) effect of genetic mutations in a zinc transporter on sphodylocheiro dysplastic form of Ehlers- Danlos Syndrome (David Eide), (xii) immune maturation and regulation in farm and non-farm raised children to better understand wheezing illnesses (David Gern), (xiii) transcription factors in tumor suppression and DNA damage (Randall Tibbets), and (xiv) development of anti-fungal vaccines (Marcel W¿thrich and Bruce Klein). Excellent institutional support in the form of 20% cost sharing of the total price of the Fortessa, salary support for trained technical staff, and the service agreement to maintain a fully functional and well-equipped cytometer will allow us to fulfill the research needs of major and minor users for the next 10 years.project_title:
Special BD LSR Fortessacontact_pi_name:
PATANKAR, MANISH Stotal_cost:
$350,505.00is_active:
Nobudget_end:
04/30/2010date_added:
03/09/2011agency_code:
NIHfiscal_year:
2009project_num:
1S10RR025483-01award_amount:
$449,000.00budget_start:
05/01/2009org_name:
UNIVERSITY OF WISCONSIN-MADISONorg_state:
WIorg_country:
UNITED STATESorg_zipcode:
537151218primary_duns:
161202122abstract_text:
DESCRIPTION (provided by applicant): This application requests funding for an LSR II flow cytometer for quantitative analysis of fluorescence in fixed and living cells examined in numerous flow cytometric assays. The instrument will be installed in the newly built Interdisciplinary Research Complex (IRC) with laboratory space designed specifically for flow cytometry and will become part of the University of Wisconsin Paul P. Carbone Comprehensive Cancer Center's flow cytometry facility, which serves investigators from multiple departments throughout the University of Wisconsin research community. The mission of the IRC is to allow new scientific discoveries to move rapidly from research laboratories to clinical care settings. It will serve physicians and scientists that study many health- related cellular mechanisms, including organ rejection, cellular interactions in asthma, mechanisms of cancer development, and drug discovery. This benchtop cytometer will be equipped with a high-throughput screening device and 405, 488, 532, and 640 nm lasers. In addition to providing new features, the cytometer will relieve the demand for the existing LSRII. This instrument will come equipped with three components not currently available on the UW campus: a 532 nm laser, a 407 nm paired with 6 photomultiplier tubes, and a high-throughput sample-delivery device that will provide users with rapid, automated sample acquisition for experiments set-up in 96- or 348-well plates. In summary, the new cytometer will serve the needs of a broad NIH-funded scientific community at the University of Wisconsin campus and will expand the current technology available for fluorescence analysis. Public Health Relevance: The multi-color flow cytometer requested will be able to measure up to 18 different molecules simultaneously in or on the cells being studied; it will support the work of many different physicians and scientists who want to begin to understand how different molecular and cellular mechanisms work together in many different types of diseases. For example, it will enable transplant surgeons to dissect cellular mechanisms of organ rejection, allergists to understand immunological mechanisms in asthma, and basic scientists to understand multiple mechanisms of cancer development and examine the effect of many different compounds in the treatment of these diseases. Placement of this instrument in the Interdisciplinary Research Complex with close proximity to the hospital will allow new scientific discoveries to move rapidly from research laboratories to clinical care settings.project_title:
Multi-color Benchtop Flow Cytometercontact_pi_name:
BUSHMAN, WADE Atotal_cost:
$449,000.00Services are offerred outside of
Consulting is offerred outside of University of Wisconsin Carbone Cancer Center
Last Updated: 04/29/2026
