Services:Mass Spectrometry+++Metabolomics Platform+++Protein Extraction/Purification+++Proteomics
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Description:Mass spectrometry using hybrid quadrupole TOF (Sciex 5600 TripleTOF) and triplequadrupole (Sciex 4000QTRAP) instruments. LC-MS proteomics, including data-dependent analysis for protein identification, and data-independent analysis (e.g., SWATH) proteomics for identification and quantitation.
Lipidomic workflows using PAL-RSI for direct infusion and MSMS/All quanrirative workflows for identification of thousands of molecular species.
This facility provides services outside its institution
This facility provides consulting outside its institution
Services:CRISPR/Cas Project Design+++Embryo Cryopreservation & Recovery Services+++Genotyping+++SgRNA Production+++Transgenics
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Description:The Mouse Transgenic Core Facility provides a series of high quality services for generating genetically modified mice. These services include nucleic aside microinjection into mouse zygotes and full service CRISPR/Cas microinjection services.
This facility provides services outside its institution
This facility does not consult outside its institution
Services:Histology+++Immunohistochemistry
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Description:The Histopathology Core facility was established to provide Histology services to the investigators associated with the Center of Biomedical Research Excellence (COBRE)in Regenerative Medicine. The objective is to provide high quality stained tissue sections on microscope slides to support the research at the institute. Services include tissue fixation, processing, embedding, sectioning, staining and coverslipping of paraffin, plastic or frozen tissue sections. Staining of tissue sections ranges from routine H&E's (Hematoxylin-Eosin) to special stains demonstrating specific tissue structures such as collagen, amyloid, bone, cartilage, vessels, kidney basement membranes, hematopoetic cells and PAS reactions. Stains offered include Trichrome, Von Kossa, PicroSirius Red, Elastic, Giemsa, PAS and others are developed and provided as needed. The Core offers antibody validation services with respect to immunohistochemistry and provides a wide range of immunohistochemical stains. In addition, the Core generates custom tissue microarrays and performs bone histomorphometry.
This facility provides services outside its institution
This facility provides consulting outside its institution
Services:Cell Imaging+++Data Analysis+++Microscopy+++Nucleic Acid Extraction+++Real-time qPCR+++RNA analysis+++RNA Integrity
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Description:The Molecular Phenotyping core provides instrumentation, training, and services for in vitro cell phenotyping. Specifically, we provide facilities and training for automated nucleic acids purification, microarray analysis, quantitative PCR, and automated immunofluorescence cell marker analysis and screening.
This facility provides services outside its institution
This facility provides consulting outside its institution
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Description:Core offers a variety of preclinical imaging services. Small animal imaging core facility with high-resolution ultrasound (FujiFilm, Vevo F2, Visual Sonics) and micro CT (SkyScan 1276 (Bruker), micro CT with in vivo and ex vivo capabilities, Caliper IVIS Lumina LT (Revvity). Offers complete services for non-destructive, longitudinal optical imaging with bioluminescence and fluorescence capabilities of mice.
This facility does not provide services outside its institution
This facility does not consult outside its institution