Production of novel monoclonal antibodies against investigator supplied immunogens using classical hybridoma technology and reverse protein microarray screens

The Jackson Laboratory Microscopy Services, located in Bar Harbor, Maine and Farmington, Connecticut

Scientific Instrument Services provides technical support functions including the repair and maintenance of lab equipment; custom design and fabrication of new fixtures and devices using CAD, 3-D printing, and traditional machining; protocol development and performance optimization of a wide variety of automated liquid handlers deployed in many of our institutions core services.

The Mass Spectrometry and Protein Chemistry (MASPC) Service aims to provide state-of-the-art mass spectrometry and protein analyses. The Mass Spectrometry side is equipped to provide high-throughput analyses for metabolomics, proteomics, single-molecule, and mass spectrometry imaging studies. The Protein Chemistry service provides extensive experience in protein analyses, including purification, antibody based detection and quantitation, gel electrophoresis, Western Blots, and more.

The Computational Sciences core is an interdisciplinary group of bioinformatics software engineers, biologists, and mathematicians providing statistical consulting, data analysis, method development, algorithm design, software engineering, high performance computing, regional compute grid development, data resources, analysis tools and tool integration, and experimental design services to biomedical research investigators at The Jackson Laboratory.

The EM Service offers both SEM (Scanning electron microscopy) and TEM (Transmission electron microscopy) as well as sample preparation for both types of EM. The EM Service is equipped with a Hitachi S3000N Variable pressure Scanning electron Microscope and a JEOL 1230 Transmission electron Microscope, both with digital image capture capability.

Reproductive Sciences is composed of Importation, Cryopreservation, Cell Biology and Microinjection groups. These groups provide a variety of services including creation of genetically modified mice, cryopreservation, recovery of cryopreserved mouse strains, rapid expansion of lines using in vitro fertilization, rederivation of imported mouse lines and maintenance of a repository of publicly distributed cryopreserved embryos, sperm and ovaries. In addition to providing these services, the Reproductive Sciences group maintains an active research program focused on the development, refinement and implementation of assisted reproductive technologies that can be used to manage strains more effectively and an education program designed to facilitate the transfer of these technologies to the scientific community.

The Glassware Service provides for cleaning and sterilizing of laboratory glassware and associated instruments. The central facility has 2 Steris Century Pre-vac Autoclaves, 2 Steris Reliance 400 Washers, several ovens, an acid-wash station, and a pipette plugger. Personnel also support the several autoclaves in shared equipment rooms throughout the laboratory complex.