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MPI AGE_FACS & Imaging Core Facility (Flow Cytometry and Microscopy)

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Joseph-Stelzmann-Str. 9b

Cologne,

50931

Germany

https://www.age.mpg.de/facs-imaging

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https://www.coremarketplace.org/MPI-AGE_FACS-Imaging https://www.coremarketplace.org/RRID:SCR_021354

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Primary Contact:

Christian Kukat

0000-0003-1508-0229

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RRID:SCR_021354

Facility Details

The FACS & Imaging Core Facility at the Max Planck Institute for Biology of Ageing in Cologne. Germany provides well-maintained state-of-the-art cytometers and cell sorters, light microscopes, and histology equipment.

Core Facility staff provide training for all users. The facility is accessible to all research groups at the Max Planck Institute for Biology of Ageing. External users may be granted access on an individual basis.

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Last Updated: 05/20/2025

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Services offered:

3D Visualization

Analysis Workstations

Flow Cytometric Analysis

Image Processing And Analysis

Immunohistochemistry

Light-sheet Microscopy

Live Cell Imaging

Multi-color Flow Cytometry

Single-Cell Analysis

Super-resolution Microscopy

Tissue Culture

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Publications

Publications associated with this facility (Click To View):

1.) Titze VM, Caixeiro S, Dinh VS, König M, Rübsam M, Pathak N, Schumacher AL, Germer M, Kukat C, Niessen CM, Schubert M, Gather MC (2024 Mar). Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers. Nature protocols, 19(3), 928-959. doi: 10.1038/s41596-023-00924-6. ID: 38238582.

2.) De Solis AJ, Del Río-Martín A, Radermacher J, Chen W, Steuernagel L, Bauder CA, Eggersmann FR, Morgan DA, Cremer AL, Sué M, Germer M, Kukat C, Vollmar S, Backes H, Rahmouni K, Kloppenburg P, Brüning JC (2024 Mar). Reciprocal activity of AgRP and POMC neurons governs coordinated control of feeding and metabolism. Nature metabolism, 6(3), 473-493. . ID: 38378998.

3.) Belkina AC, Roe CE, Tang VA, Back JB, Bispo C, Conway A, Chakraborty U, Daniels KT, de la Cruz G, Ferrer-Font L, Filby A, Gravano DM, Gregory MD, Hall C, Kukat C, Mozes A, Ordoñez-Rueda D, Orlowski-Oliver E, Pesce I, Porat Z, Poulton NJ, Reifel KM, Rieger AM, Sheridan RTC, Van Isterdael G, Walker RV (2024 Feb). Guidelines for establishing a cytometry laboratory. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 105(2), 88-111. doi: 10.1002/cyto.a.24807. ID: 37941128.

4.) Vossen C, Schmidt P, Wunderlich CM, Mittenbühler MJ, Tapken C, Wienand P, Mirabella PN, Cabot L, Schumacher AL, Folz-Donahue K, Kukat C, Voigt I, Brüning JC, Fenselau H, Wunderlich FT (2024 Jan 4). An Approach to Intersectionally Target Mature Enteroendocrine Cells in the Small Intestine of Mice. Cells, 13(1), . . ID: 38201306.

5.) Kukat C, Neumann E, Fava E, Fried H (2024 Jan). A novel room concept for shared resource laboratories. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 105(1), 16-23. doi: 10.1002/cyto.a.24795. ID: 37772729.

6.) Nikopoulou C, Kleinenkuhnen N, Parekh S, Sandoval T, Ziegenhain C, Schneider F, Giavalisco P, Donahue KF, Vesting AJ, Kirchner M, Bozukova M, Vossen C, Altmüller J, Wunderlich T, Sandberg R, Kondylis V, Tresch A, Tessarz P (2023 Nov). Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver. Nature aging, 3(11), 1430-1445. . ID: 37946043.

7.) Tschernoster N, Erger F, Kohl S, Reusch B, Wenzel A, Walsh S, Thiele H, Becker C, Franitza M, Bartram MP, Kömhoff M, Schumacher L, Kukat C, Borodina T, Quedenau C, Nürnberg P, Rinschen MM, Driller JH, Pedersen BP, Schlingmann KP, Hüttel B, Bockenhauer D, Beck B, Altmüller J (2023 Aug 23). Long-read sequencing identifies a common transposition haplotype predisposing for CLCNKB deletions. Genome medicine, 15(1), 62. . ID: 37612755.

8.) Rivera-Mejías P, Narbona-Pérez ÁJ, Hasberg L, Kroczek L, Bahat A, Lawo S, Folz-Donahue K, Schumacher AL, Ahola S, Mayer FC, Giavalisco P, Nolte H, Lavandero S, Langer T (2023 Apr 25). The mitochondrial protease OMA1 acts as a metabolic safeguard upon nuclear DNA damage. Cell reports, 42(4), 112332. doi: 10.1016/j.celrep.2023.112332. ID: 37002921.

9.) Vesting AJ, Jais A, Klemm P, Steuernagel L, Wienand P, Fog-Tonnesen M, Hvid H, Schumacher AL, Kukat C, Nolte H, Georgomanolis T, Altmüller J, Pasparakis M, Schmidt A, Krüger M, Supprian MS, Waisman A, Straub BK, Raschzok N, Bernier M, Birkenfeld AL, Hövelmeyer N, Brüning JC, Wunderlich FT (2022 Dec). NIK/MAP3K14 in hepatocytes orchestrates NASH to hepatocellular carcinoma progression via JAK2/STAT5 inhibition. Molecular metabolism, 66(), 101626. . ID: 36356831.

10.) Gehling K, Parekh S, Schneider F, Kirchner M, Kondylis V, Nikopoulou C, Tessarz P (2022 Aug 1). RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variability. Life science alliance, 5(12), . . ID: 35914813.

11.) Tschernoster N, Erger F, Walsh PR, McNicholas B, Fistrek M, Habbig S, Schumacher AL, Folz-Donahue K, Kukat C, Toliat MR, Becker C, Thiele H, Kavanagh D, Nürnberg P, Beck BB, Altmüller J (2022 Jun). Unraveling Structural Rearrangements of the CFH Gene Cluster in Atypical Hemolytic Uremic Syndrome Patients Using Molecular Combing and Long-Fragment Targeted Sequencing. The Journal of molecular diagnostics : JMD, 24(6), 619-631. doi: 10.1016/j.jmoldx.2022.02.006. ID: 35398599.

12.) Sun H, Jiao WB, Krause K, Campoy JA, Goel M, Folz-Donahue K, Kukat C, Huettel B, Schneeberger K (2022 Mar). Chromosome-scale and haplotype-resolved genome assembly of a tetraploid potato cultivar. Nature genetics, 54(3), 342-348. . ID: 35241824.

13.) Boehm U, Nelson G, Brown CM, Bagley S, Bajcsy P, Bischof J, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Nitschke R (2022 Feb). Author Correction: QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. Nature methods, 19(2), 256. doi: 10.1038/s41592-021-01387-x. ID: 34980905.

14.) Pouikli A, Parekh S, Maleszewska M, Nikopoulou C, Baghdadi M, Tripodi I, Folz-Donahue K, Hinze Y, Mesaros A, Hoey D, Giavalisco P, Dowell R, Partridge L, Tessarz P (2021 Sep). Chromatin remodeling due to degradation of citrate carrier impairs osteogenesis of aged mesenchymal stem cells. Nature aging, 1(9), 810-825. . ID: 37117628.

15.) Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker GJ, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen CL, (2021 Jul 2). QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of microscopy, (), . doi: 10.1111/jmi.13041. ID: 34214188.

16.) Damen M, Wirtz L, Soroka E, Khatif H, Kukat C, Simons BD, Bazzi H (2021 May 28). High proliferation and delamination during skin epidermal stratification. Nature communications, 12(1), 3227. doi: 10.1038/s41467-021-23386-4. ID: 34050161.

17.) Boehm U, Nelson G, Brown CM, Bagley S, Bajcsy P, Bischof J, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Nitschke R (2021 May 21). QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. Nature methods, (), . doi: 10.1038/s41592-021-01162-y. ID: 34021279.

18.) Campoy JA, Sun H, Goel M, Jiao WB, Folz-Donahue K, Wang N, Rubio M, Liu C, Kukat C, Ruiz D, Huettel B, Schneeberger K (2020 Dec 29). Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes. Genome biology, 21(1), 306. doi: 10.1186/s13059-020-02235-5. ID: 33372615.

19.) Dietzel S, Ferrando-May E, Fried H, Kukat C, Naumann A, Nitschke R, Pasierbek P, Peychl J, Rasse TM, Schroth-Diez B, Stöckl MT, Terjung S, Thuenauer R, Tulok S, Weidtkamp-Peters S (2020 Sep). A Joint Action in Times of Pandemic: The German BioImaging Recommendations for Operating Imaging Core Facilities During the SARS-Cov-2 Emergency. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(9), 882-886. doi: 10.1002/cyto.a.24178. ID: 32583531.

20.) Motori E, Atanassov I, Kochan SMV, Folz-Donahue K, Sakthivelu V, Giavalisco P, Toni N, Puyal J, Larsson NG (2020 Aug). Neuronal metabolic rewiring promotes resilience to neurodegeneration caused by mitochondrial dysfunction. Science advances, 6(35), eaba8271. . ID: 32923630.

21.) Jiang H, Gallet S, Klemm P, Scholl P, Folz-Donahue K, Altmüller J, Alber J, Heilinger C, Kukat C, Loyens A, Müller-Fielitz H, Sundaram S, Schwaninger M, Prevot V, Brüning JC (2020 Jul 22). MCH Neurons Regulate Permeability of the Median Eminence Barrier. Neuron, 107(2), 306-319.e9. . ID: 32407670.

22.) Krämer M, Plum PS, Velazquez Camacho O, Folz-Donahue K, Thelen M, Garcia-Marquez I, Wölwer C, Büsker S, Wittig J, Franitza M, Altmüller J, Löser H, Schlößer H, Büttner R, Schröder W, Bruns CJ, Alakus H, Quaas A, Chon SH, Hillmer AM (2020 Jun). Cell type-specific transcriptomics of esophageal adenocarcinoma as a scalable alternative for single cell transcriptomics. Molecular oncology, 14(6), 1170-1184. doi: 10.1002/1878-0261.12680. ID: 32255255.

23.) Gӧbel J, Engelhardt E, Pelzer P, Sakthivelu V, Jahn HM, Jevtic M, Folz-Donahue K, Kukat C, Schauss A, Frese CK, Giavalisco P, Ghanem A, Conzelmann KK, Motori E, Bergami M (2020 Apr 7). Mitochondria-Endoplasmic Reticulum Contacts in Reactive Astrocytes Promote Vascular Remodeling. Cell metabolism, 31(4), 791-808.e8. . ID: 32220306.

24.) Baumgart J, Kirchner M, Redemann S, Bond A, Woodruff J, Verbavatz JM, Jülicher F, Müller-Reichert T, Hyman AA, Brugués J (2019 Dec 2). Soluble tubulin is significantly enriched at mitotic centrosomes. The Journal of cell biology, 218(12), 3977-3985. . ID: 31636117.

25.) Cossarizza A, Chang HD, Radbruch A, Acs A, Adam D, Adam-Klages S, Agace WW, Aghaeepour N, Akdis M, Allez M, Almeida LN, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari CT, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgart S, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman DA, Bogdan C, Borger JG, Borsellino G, Boulais PE, Bradford JA, Brenner D, Brinkman RR, Brooks AES, Busch DH, Büscher M, Bu (2019 Oct). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European journal of immunology, 49(10), 1457-1973. doi: 10.1002/eji.201970107. ID: 31633216.

26.) Czechowska K, Lannigan J, Wang L, Arcidiacono J, Ashhurst TM, Barnard RM, Bauer S, Bispo C, Bonilla DL, Brinkman RR, Cabanski M, Chang HD, Chakrabarti L, Chojnowski G, Cotleur B, Degheidy H, Dela Cruz GV, Eck S, Elliott J, Errington R, Filby A, Gagnon D, Gardner R, Green C, Gregory M, Groves CJ, Hall C, Hammes F, Hedrick M, Hoffman R, Icha J, Ivaska J, Jenner DC, Jones D, Kerckhof FM, Kukat C, Lanham D, Leavesley S, Lee M, Lin-Gibson S, Litwin V, Liu Y, Molloy J, Moore JS, Müller S, Nedbal J, Ni (2019 Jun). Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(6), 598-644. doi: 10.1002/cyto.a.23777. ID: 31207046.

27.) Vogel S, Schäfer C, Hess S, Folz-Donahue K, Nelles M, Minassian A, Schwarz MK, Kukat C, Ehrlich M, Zaehres H, Kloppenburg P, Hoehn M, Aswendt M (2019 May). The in vivo timeline of differentiation of engrafted human neural progenitor cells. Stem cell research, 37(), 101429. . ID: 30933718.

28.) Galvin A, Weglarz M, Folz-Donahue K, Handley M, Baum M, Mazzola M, Litwa H, Scadden DT, Silberstein L (2019 Jan). Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry. Current protocols in cytometry, 87(1), e50. doi: 10.1002/cpcy.50. ID: 30335223.

29.) Collmann FM, Pijnenburg R, Hamzei-Taj S, Minassian A, Folz-Donahue K, Kukat C, Aswendt M, Hoehn M (2019). Individual <i>in vivo</i> Profiles of Microglia Polarization After Stroke, Represented by the Genes iNOS and Ym1. Frontiers in immunology, 10(), 1236. doi: 10.3389/fimmu.2019.01236. ID: 31214190.

30.) Collmann FM, Pijnenburg R, Schneider G, Schäfer C, Folz-Donahue K, Kukat C, Hoehn M (2018 Sep). Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 13(3), 371-382. doi: 10.1007/s11481-018-9789-2. ID: 29790106.

31.) Kauppila JHK, Bonekamp NA, Mourier A, Isokallio MA, Just A, Kauppila TES, Stewart JB, Larsson NG (2018 Jul 27). Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice. Nucleic acids research, 46(13), 6642-6669. . ID: 29860357.

32.) Jiang M, Kauppila TES, Motori E, Li X, Atanassov I, Folz-Donahue K, Bonekamp NA, Albarran-Gutierrez S, Stewart JB, Larsson NG (2017 Aug 1). Increased Total mtDNA Copy Number Cures Male Infertility Despite Unaltered mtDNA Mutation Load. Cell metabolism, 26(2), 429-436.e4. . ID: 28768180.

33.) Theurich S, Tsaousidou E, Hanssen R, Lempradl AM, Mauer J, Timper K, Schilbach K, Folz-Donahue K, Heilinger C, Sexl V, Pospisilik JA, Wunderlich FT, Brüning JC (2017 Jul 5). IL-6/Stat3-Dependent Induction of a Distinct, Obesity-Associated NK Cell Subpopulation Deteriorates Energy and Glucose Homeostasis. Cell metabolism, 26(1), 171-184.e6. . ID: 28683285.

34.) Cheng C, Fass DM, Folz-Donahue K, MacDonald ME, Haggarty SJ (2017 Jan 11). Highly Expandable Human iPS Cell-Derived Neural Progenitor Cells (NPC) and Neurons for Central Nervous System Disease Modeling and High-Throughput Screening. Current protocols in human genetics, 92(), 21.8.1-21.8.21. doi: 10.1002/cphg.33. ID: 28075486.

35.) Ferrando-May E, Hartmann H, Reymann J, Ansari N, Utz N, Fried HU, Kukat C, Peychl J, Liebig C, Terjung S, Laketa V, Sporbert A, Weidtkamp-Peters S, Schauss A, Zuschratter W, Avilov S (2016 Jun). Advanced light microscopy core facilities: Balancing service, science and career. Microscopy research and technique, 79(6), 463-79. doi: 10.1002/jemt.22648. ID: 27040755.

36.) Kukat C, Davies KM, Wurm CA, Spåhr H, Bonekamp NA, Kühl I, Joos F, Polosa PL, Park CB, Posse V, Falkenberg M, Jakobs S, Kühlbrandt W, Larsson NG (2015 Sep 8). Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid. Proceedings of the National Academy of Sciences of the United States of America, 112(36), 11288-93. . ID: 26305956.

37.) Lagouge M, Mourier A, Lee HJ, Spåhr H, Wai T, Kukat C, Silva Ramos E, Motori E, Busch JD, Siira S, Kremmer E, Filipovska A, Larsson NG (2015 Aug). SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation. PLoS genetics, 11(8), e1005423. doi: 10.1371/journal.pgen.1005423. ID: 26247782.

38.) Baggio F, Bratic A, Mourier A, Kauppila TE, Tain LS, Kukat C, Habermann B, Partridge L, Larsson NG (2014 Dec 16). Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation. Nucleic acids research, 42(22), 13920-38. . ID: 25428350.

39.) Kühl I, Kukat C, Ruzzenente B, Milenkovic D, Mourier A, Miranda M, Koolmeister C, Falkenberg M, Larsson NG (2014 Oct 9). POLRMT does not transcribe nuclear genes. Nature, 514(7521), E7-11. . ID: 25297440.

40.) Kukat C, Larsson NG (2013 Sep). mtDNA makes a U-turn for the mitochondrial nucleoid. Trends in cell biology, 23(9), 457-63. . ID: 23721879.

41.) Terzioglu M, Ruzzenente B, Harmel J, Mourier A, Jemt E, López MD, Kukat C, Stewart JB, Wibom R, Meharg C, Habermann B, Falkenberg M, Gustafsson CM, Park CB, Larsson NG (2013 Apr 2). MTERF1 binds mtDNA to prevent transcriptional interference at the light-strand promoter but is dispensable for rRNA gene transcription regulation. Cell metabolism, 17(4), 618-26. . ID: 23562081.

42.) Wredenberg A, Lagouge M, Bratic A, Metodiev MD, Spåhr H, Mourier A, Freyer C, Ruzzenente B, Tain L, Grönke S, Baggio F, Kukat C, Kremmer E, Wibom R, Polosa PL, Habermann B, Partridge L, Park CB, Larsson NG (2013). MTERF3 regulates mitochondrial ribosome biogenesis in invertebrates and mammals. PLoS genetics, 9(1), e1003178. doi: 10.1371/journal.pgen.1003178. ID: 23300484.

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Associations

ISAC

International Society for Advancement of Cytometry

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Metadata

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https://www.coremarketplace.org/?FacilityID=1134
https://www.coremarketplace.org/RRID:SCR_021354

Institution

Institution ROR ID: https://ror.org/04xx1tc24

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Keywords:

USEDit, ABRF, FACS, Imaging, wide field microscopes, confocal microscopes, flow cytometry analyzers, flow cytometry sorters, histology equipment, PC workstations, data analysis, training

Resource Type:

access service resource, core facility, service resource

Citation:

Max Planck Institute for Biology of Ageing FACS and Imaging Core Facility (RRID:SCR_021354)

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MPI AGE_FACS & Imaging Core Facility (Flow Cytometry and Microscopy)

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