The Advanced Light Microscopy Unit (ALMU) is a walk-in core unit on a mission to help, support and educate the research community from CRG and beyond to do quantitative bioimaging. We provide support on a range of technologies starting from experimental design, sample preparation, acquisition, bioimage analysis and visualization. Our unit consists of microscope systems specifically designed for fluorescence microscopy ranging from confocal, widefield to super-resolution systems and helps reveal structures from macro to the nanoscale.

The Bioinformatics Unit provides researchers at CRG-CNAG/PRBB and external organizations with services of consultation, planning NGS and other genomic experiments, NGS data processing, analysis and management, software and database development, bioinformatics training, and access to high-performance computing resources at CRG. The Unit works in synergy with the Genomics Unit and the Biomolecular Screening & Protein Technologies Unit (BMS-PT) to support users using high-throughput sequencing technologies from an experiment planning to delivery of timely and reliable results.

The Protein Technologies Unit provides the researchers at the CRG-CNAG/PRBB, external institutions and private companies with a new, high quality technology platform to perform protein production and automated processes in the context of biomolecular and biochemical screening assays. Through staff-assisted screening, users will have access to the equipment of the facility including liquid handlers, plate readers and the small molecule libraries. In addition, the unit is aimed at supporting the researchers of the CRG institute by purchasing, seeking and maintaining valuable stocks of clones and strains.

Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest. The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows. The Unit currently hosts five analyzers and two sorters, and is therewith the largest Becton Dickinson site in Spain. The sorters are operated by the facility personnel, who also support the users in experimental design and data analysis. The analyzers are operated by the users themselves, after they have been trained. The facility personnel helps with the experimental design and supervises and monitors the experiments when necessary.

The Proteomics Unit is a joint effort of the Universitat Pompeu Fabra and the Center of Genomic Regulation to create an innovative research infrastructure with a high-level of excellence and sustainability to support scientists in the biomedical field. The activities of the Proteomics Unit are focused onto the provision of high-quality proteomics services with strong added-value to the research community by i) offering state-of-the-art methods, advise and expertise to support basic and translational researchers; ii) developing new methods and techniques that keep the unit up to date and at the forefront of the proteomics field; and iii) training the community and actively disseminating proteomics science and methods. The Proteomics Unit is part of the Spanish National Infrastructure for Omics Technologies (ICTS OmicsTech) and the European Proteomics Infrastructure Consortium (EPIC-XS, GA 823839), and it coordinates the European innovative and interdisciplinary Research and Training network PROTrEIN (GA 956148). The Proteomics Unit is also part of the "Plataforma de Recursos Biomoleculares y Bioinformáticos - ProteoRed" of the Instituto de Salud Carlos III, part of the Spanish National Health System (PT17/0019 ).

The CRG Genomics Core Facility was established in 2008 and currently supports next generation sequencing technologies. Since its foundation, the CRG has made significant investments in Genomics, including the purchase of several next generation sequencing instruments. Several high-throughput technologies have been implemented to assess differential expression, inter-individual genetic variation, microRNA discovery, genome sequencing, targeted resequencing, epigenetic profiling and identification of binding sites of DNA or RNA-associated proteins.