The University of Washington Mouse CRISPR Core (UWMCC) is a cost center administered through the Department of Comparative Medicine at the University of Washington (UW), Seattle, WA. The UWMCC is a full-service facility offering rapid, precise and economical generation of novel transgenic mouse models using CRISPR/Cas9 gene editing technology. Our standard service includes consultation, design, testing, prep, mouse embryo microinjection, and genotyping all at a competitive price point. The UWMCC is a joint collaboration utilizing 15 years of gene-editing experience by the UW Transgenic Resources Program, the UW Institute for Stem Cell and Regenerative Medicine, and the Vector and Transgenic Mouse Core of the UW Diabetes Research Center.

The Transgenic Resources Program (TRP) is a core facility that creates transgenic and gene-targeted mice using pronuclear microinjection, targeted ES cell microinjection, and CRISPR-Cas9 gene editing. The TRP also offers mouse rederivation services to create specific pathogen free mice or to revive cryopreserved mouse lines. Additionally, embryo and sperm cryopreservation services are available to provide long-term storage of valuable mouse strains or stocks. Our facility is available to all University of Washington investigators and outside institutions.

Magnetoencephalography facility focusing on children. Equipped with MEGIN TRIUX Neo system with helium recycler, MAGSTIM EEG data acquisition system, Tucker-Davis Technologies RZ6 for stimulus generation, Epson Home Cinema 3200 for video projection, custom video recording system with 4 cameras, Eyelink eyetracker, and Polhemus 3D digitizer.

The Preclinical Research and Translational Services (PRTS) Core at the University of Washington (UW) is a collection of individual core facilities that provide support for both internal (UW) and external (outside of UW) collaborators, researchers, and scientists.

Managed by the Department of Comparative Medicine, the PRTS Core provides in-life services including the Transgenic Resources Program (TRP), In Vivo Services (IVS), the Gnotobiotic Animal Core (GNAC), the Biosafety Level (BSL-3) Facility, and the Patient Derived Xenograft (PDX) Core, as well as pathology support by the Histology and Imaging Core (HIC) and the Comparative Pathology Program (CPP).

The Gnotobiotic Animal Core (GNAC) at the University of Washington (UW) is a core facility started in 2014 and managed by the Department of Comparative Medicine. Located in the UW Health Sciences Center, the GNAC is open to investigators with research needs to create or maintain mice free of any microorganisms (germ-free), or with a known population of microorganisms (gnotobiotic). Our facility is available to all University of Washington investigators as well as outside institutions.

The Histology and Imaging Core (HIC) provides histology, immunohistochemistry, imaging, quantitative image analysis, and pathology consultation services. We employ extensive experience, expertise and state of the art high-throughput instrumentation across all platforms in our facility. This core is open to all investigators, including; UW and their affiliates, commercial and not-for-profit biotechnology companies, and other academic institutions. Through our partnership with the Comparative Pathology Program (CPP) we have access to experienced comparative pathologists who are available for research consultation and collaboration.

The In Vivo Services (IVS) Core provides mouse colony management at the UW. IVS staff will oversee the maintenance, breeding, weaning, identification, and record keeping of your mouse colonies to free up the limited and valuable time of your laboratory personnel. In addition, the IVS Core offers an array of technical services performing procedures such as sample collection, sample administration, metabolic testing, behavioral assays, and testing of drug compounds in rodents. Our facility is available to all University of Washington investigators and outside institutions.

The Sanford Burnham Prebys Cancer Metabolism Core provides a unique service for the analysis of metabolism, offering both metabolite quantification and stable-isotope tracing methodology, centered around gas chromatography-mass spectrometry (GC-MS). We offer GC-MS analysis of polar metabolites (TCA cycle, glycolysis metabolites and amino acids), short chain fatty acids, fatty acids and cholesterol, sugars and sugar-phosphates. As well as cells, we can also analyze tissues, tumors, serum or culture medium as necessary. We also have available a YSI 2950 analyzer for measurement of glucose, lactate and glutamine in medium samples. We collaborate closely with Core users and can provide in-depth advice on the direction of metabolic experiments, as well as the interpretation of results.

The Sanford-Burnham Proteomics Facility provides state-of-the-art mass spectrometry-based proteomics services with methods tailored to the needs of scientific investigators for routine mass spectrometric analysis of peptides and proteins, identification of novel proteins, characterization of post-translational modifications, and quantitative proteomics analysis of simple to complex biological samples. The facility identifies, validates and develops new methods designed for the characterization and identification of biologically important proteins. It also supports users with software applications and development, data analysis and experimental design of proteomics studies. The facility is continuously expanding its capabilities in large-scale differential proteomics analysis and phosphoproteomics for unbiased discovery of potential biomarkers and mapping protein profiles. We provide proteomics services to Sanford-Burnham scientists as well as outside non-profit and for-profits organization on a fee-for-service basis.

A flow and mass cytometry core for the University of Maryland system.

The Protein Analysis Lab provides multiple technologies for the purification and analysis of proteins, peptides and small molecules. Mass Spectrometry is used for protein identification, quantitative global protein profiling and targeted quantification of peptides and small molecules. HPLC techniques are used for analysis or purification of biomolecules and include ion exchange, hydrophilic interaction, size-exclusion, and reversed phase chromatography.

SBMRI Proteomics Facility provides state-of-the-art mass spectrometry (MS)-based proteomic services for analysis of peptides, synthetic compounds, carbohydrates and proteins by facilitating protein identification, the qualitative and quantitative characterization of post-translational modifications, plus quantitative and differential proteomic profiling of low-complex biological samples. Facility staff regularly identifies, validates, develops, and implements new methodologies for identification, quantification and characterization of biologically important proteins. Such approaches can support a mechanistic understanding of cancer and other life threatening diseases, as well as identify new therapeutic targets and diagnostic biomarkers.
The Proteomics Facility also supports users with grant write-up, proteomics software applications, data analysis, and experimental design of proteomic studies. The facility is continuously expanding its capabilities in large-scale differential proteomics analysis and in phosphoproteomics (and other PTMs) for discovery of potential biomarkers and mapping protein profiles.

State-of-the-art imaging and analytical services:
- The facility can perform in vivo non-invasive luminescence and fluorescence imaging for xenograft tumor growth and metastasis studies.

- Analytical equipment supports complete blood cell counts (CBC) and analysis of serum components revealing metabolic or organ stress from small samples of mouse blood.

The animal facility provides housing for specific pathogen free (SPF) rodents, frogs and Zebrafish. Trained animal care technicians provide expertise in animal husbandry, transgenic and knockout mouse breeding colony maintenance and assistance with routine technical procedures.

Our animal research program is accredited by AAALAC International (the Association for Assessment and Accreditation of Laboratory Animal Care International. Participating in the AAALAC accreditation program and meeting their rigorous standards demonstrates our commitment to humane and responsible animal research and our dedication to good science

Variety of protein production services including cloning, expression optimization, development of protein purification protocols, and protein preparation. E. coli, insect (SF9, baculovirus) and mammalian (293) systems are available for protein expression. Our analytical services are focused on biophysical characterization of structural and functional properties of proteins in solution, under native, non-denaturing conditions. We can perform quality control of protein samples (folding, stability, aggregation, etc.); characterize protein conformation and shape in solution; and measure protein binding to proteins, peptides, small molecules and other ligands (including determination of equilibrium (Kd) and kinetic rate (kon, koff) constants, stoichiometry, binding enthalpy and entropy).

The Stem Cell Core Facility (SCCF) at the Burnham Institute provides scientist’s within the Sanford Stem Cell Consortium an area to conduct their research that is devoted entirely to Human stem cells and that is conducive to collaborative partnerships.
The SSCF also provides researchers with a source of both approved and non-approved embryonic stem cell lines, induced pluripotent stem cell’s, cell-line profiling services, reagents, and instruction for all areas regarding the maintenance, differentiation and manipulation of stem cells. The SCCF has also just completed a CIRM funded renovation and expansion that allows us to increase greatly the products and services that we can offer the members of the Consortium.

The facility provides microarray-based global analysis of gene expression in human, mice and rats using an Illumina platform. Starting with investigator RNA samples, the facility confirms RNA quality, and then performs microarray analysis as well as initial data analysis. Array data are returned to the investigator, and if desired, also passed on to the Informatics and Data Management facility for more detailed analysis. Both mRNA and miRNA profiling are available. Quantitative real-time PCR (Q-PCR) service is predominantly SYBR green-based analysis, but hybridization probe (e.g. TaqMan) approaches are supported. Starting with RNA, the facility will utilize primers from investigators, or design custom primers and perform Q-PCR. The facility provides control primers and assists with experimental design, as well as providing basic relative quantitation analysis. Q-PCR analysis in the facility can also provide gene, RNA, or viral copy number data.

The Histopathology Core Facility provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. The Facility also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. The Facility maintains and provides training for several pieces of common use equipment, such as, paraffin microtome, cryostat, and a cytospin. Training is also provided for basic histological techniques such as, perfusion and tissue collection. Finished slides are returned to the researcher for evaluation, or they may be scanned in our Aperio Scanscopes that allows digitizing of slides for archiving, and electronic distribution/remote analysis of histology slides, and offer, powerful image analysis tools. Scanning of fluorescent slides is also available.

The Cell Imaging Facility broadly supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance, helping scientists from any institution on a “first come-first served” basis.

It offers expertise, training and assistance in advanced biological microscopic imaging techniques and use of complex image processing software, use of well-maintained, aligned, and calibrated microscopic equipment, and troubleshooting of equipment and experimental problems.

The Microarray Facility provides services and support for researchers doing RNA expression, miRNA expression, transcription factor binding and other assays utilizing microarray technology. Services are available to all researchers at the Institute as well as external institutions and collaborators. The facility supports the Affymetrix, Agilent, and Exiqon platforms for RNA and miRNA measurements as well as an in-house spotter to fabricate custom oligo arrays. Also available is the Fluidigm Biomark which can run single cell expression analysis.

Provides services in the conduct of exercise interventions, nutrition interventions, or prevention-based clinical interventions. Facility offers well-trained, specialized staff and inviting space for study participants to participate in population-based research.

Leica Microsystems and Fred Hutch collaborated to establish the first Leica Center of Excellence, or CoE, in the Pacific Northwest. The Leica CoE establishes a mutually beneficial, collaborative relationship through a combined effort to provide researchers with advanced microscopy technologies, while supporting the Hutch’s commitment to generating scientific discoveries and developing new approaches to preventing and treating cancer and related diseases.

The Therapeutic Products shared resource at Fred Hutchinson Cancer Center provides facilities and services in process development and manufacturing of Good Manufacturing Practice (GMP)-grade biologic and cellular therapeutic products for Phase 1/2 clinical trials. Our vision is to support the translation of ideas from academic laboratories to clinical trials.

Through Shared Resources, investigators in Fred Hutch/University of Washington Cancer Consortium and external academic and industry organizations can access services tailored to their specific research goals, including novel assay development or early access to new technologies.

Since our inception in 1995, Fred Hutch’s Antibody Technology shared resource has worked with research teams inside and outside the Hutch to design campaign strategies for the generation and identification of novel monoclonal antibody compositions. Our projects encompass a range of applications in oncology, viral therapeutics and diagnostics.

The Cellular Imaging shared resource at Fred Hutch provides advanced light microscopy services for biomedical and translational research. Our expert staff provides a complete workflow, including training for independent, 24/7 work in the core; assistance and technical support in microscopy techniques and their application; and tailored quantitative image analysis. We also offer training and support for imaging and analyzing gels and blots. We work with Fred Hutch users as well as external users from academia and industry.

Fred Hutch and Leica Microsystems have combined efforts to establish a Leica Center of Excellence in the Cellular Imaging core in support of a mission to drive new discoveries and insights from scientific research performed using the imaging systems.

The Comparative Medicine shared resource provides a variety of research support services. All our facilities are fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) International and comply with all U.S. Department of Agriculture, Public Health Service, Washington state and local area regulations.

Offers techniques to resolve structures from cellular ultrastructure to molecular interactions. Offers expertise in EM sample preparation and imaging, cryogenic electron microscopy, technologies and services, training through complexities of electron microscopy workflow, from sample preparation to image processing. All users must be trained by core staff.

Provides expertise in anatomic pathology technologies. Offers routine and special staining,  immunohistochemistry, in situ hybridization and digital pathology in support of internal and external investigators who rely on histological analysis.

Includes services from sterile cell sorting for therapeutic applications to whole cell mass spectrometry, diagnostics and early stage clinical trials. Provides expertise from experimental design to data analysis and troubleshooting, therapeutic cell sorting. Supports isolation of cells for Phase 1 clinical trials of cellular immunotherapies.

The Hutch Data Core helps investigators integrate, interpret and explore large and diverse datasets. We provide services that support efficient and reproducible analysis of large-scale datasets for biomedical researchers, drawing on deep expertise in computational biology, software engineering and cloud architecture.
We aim to make it possible for all interested investigators to apply advanced analytical techniques to complex datasets. Our diverse team offers project-based scientific analysis by expert bioinformaticians, automation of analytical pipelines using modern workflow management platforms, and publication of complex datasets with web-accelerated graphics.
In addition to direct bioinformatics support on a per-project basis, the Hutch Data Core offers training and documentation to enable scientist-driven computation. We also support automation of complex workflows for high-performance computing and provide access to web platforms specifically designed to meet researchers’ unique needs.

The Immune Monitoring shared resource supports investigators seeking precise evaluations of immunologic responses in both basic and early-stage clinical research settings. We provide users with resources and expertise to evaluate cellular immunologic processes and responses through reagents, molecular and cellular assays, assay development support and research-consulting services.

Provides in vivo imaging technology and infrastructure to support basic and preclinical research. Offers diverse array of imaging modalities, including ultrasound and optical imaging, MRI, micro-CT and multiphoton microscopy.

Provides support for mass spectrometry based proteomics and metabolomics research. With advanced instrumentation and highly trained staff, core offers consultation and services in sample preparation, sample separation, data collection and data analysis.

Supports development and maintenance of preclinical mouse models, including patient derived xenografts, cell line xenografts and genetically engineered mouse models.

The Genomics & Bioinformatics shared resource provides an integrated, streamlined and expertly supported set of services that encompass experimental design, data generation and data interpretation. Expert genomics and bioinformatics teams are available to consult and collaborate at every step of the process and work closely together to develop optimized experimental strategies to ensure that appropriate technology and data analysis tools are used to address each unique scientific question.

Provides biospecimen sample processing, extraction and biorepository services. Offers expertise to advance preclinical and clinical studies.

Core dedicated to the protocol driven collection, analysis, and reporting of behavioral data using a blend of classic and innovative assays. Supports neuroscience, psychology, pharmacology, genetics, cancer, and development by providing advanced tools and expertise for the precise measurement and interpretation of behavior.