The University of Washington Mouse CRISPR Core (UWMCC) is a cost center administered through the Department of Comparative Medicine at the University of Washington (UW), Seattle, WA. The UWMCC is a full-service facility offering rapid, precise and economical generation of novel transgenic mouse models using CRISPR/Cas9 gene editing technology. Our standard service includes consultation, design, testing, prep, mouse embryo microinjection, and genotyping all at a competitive price point. The UWMCC is a joint collaboration utilizing 15 years of gene-editing experience by the UW Transgenic Resources Program, the UW Institute for Stem Cell and Regenerative Medicine, and the Vector and Transgenic Mouse Core of the UW Diabetes Research Center.

The Transgenic Resources Program (TRP) is a core facility that creates transgenic and gene-targeted mice using pronuclear microinjection, targeted ES cell microinjection, and CRISPR-Cas9 gene editing. The TRP also offers mouse rederivation services to create specific pathogen free mice or to revive cryopreserved mouse lines. Additionally, embryo and sperm cryopreservation services are available to provide long-term storage of valuable mouse strains or stocks. Our facility is available to all University of Washington investigators and outside institutions.

Magnetoencephalography facility focusing on children. Equipped with MEGIN TRIUX Neo system with helium recycler, MAGSTIM EEG data acquisition system, Tucker-Davis Technologies RZ6 for stimulus generation, Epson Home Cinema 3200 for video projection, custom video recording system with 4 cameras, Eyelink eyetracker, and Polhemus 3D digitizer.

The Preclinical Research and Translational Services (PRTS) Core at the University of Washington (UW) is a collection of individual core facilities that provide support for both internal (UW) and external (outside of UW) collaborators, researchers, and scientists.

Managed by the Department of Comparative Medicine, the PRTS Core provides in-life services including the Transgenic Resources Program (TRP), In Vivo Services (IVS), the Gnotobiotic Animal Core (GNAC), the Biosafety Level (BSL-3) Facility, and the Patient Derived Xenograft (PDX) Core, as well as pathology support by the Histology and Imaging Core (HIC) and the Comparative Pathology Program (CPP).

The Gnotobiotic Animal Core (GNAC) at the University of Washington (UW) is a core facility started in 2014 and managed by the Department of Comparative Medicine. Located in the UW Health Sciences Center, the GNAC is open to investigators with research needs to create or maintain mice free of any microorganisms (germ-free), or with a known population of microorganisms (gnotobiotic). Our facility is available to all University of Washington investigators as well as outside institutions.

The Histology and Imaging Core (HIC) provides histology, immunohistochemistry, imaging, quantitative image analysis, and pathology consultation services. We employ extensive experience, expertise and state of the art high-throughput instrumentation across all platforms in our facility. This core is open to all investigators, including; UW and their affiliates, commercial and not-for-profit biotechnology companies, and other academic institutions. Through our partnership with the Comparative Pathology Program (CPP) we have access to experienced comparative pathologists who are available for research consultation and collaboration.

The In Vivo Services (IVS) Core provides mouse colony management at the UW. IVS staff will oversee the maintenance, breeding, weaning, identification, and record keeping of your mouse colonies to free up the limited and valuable time of your laboratory personnel. In addition, the IVS Core offers an array of technical services performing procedures such as sample collection, sample administration, metabolic testing, behavioral assays, and testing of drug compounds in rodents. Our facility is available to all University of Washington investigators and outside institutions.

Provides services in the conduct of exercise interventions, nutrition interventions, or prevention-based clinical interventions. Facility offers well-trained, specialized staff and inviting space for study participants to participate in population-based research.

Leica Microsystems and Fred Hutch collaborated to establish the first Leica Center of Excellence, or CoE, in the Pacific Northwest. The Leica CoE establishes a mutually beneficial, collaborative relationship through a combined effort to provide researchers with advanced microscopy technologies, while supporting the Hutch’s commitment to generating scientific discoveries and developing new approaches to preventing and treating cancer and related diseases.

The Therapeutic Products shared resource at Fred Hutchinson Cancer Center provides facilities and services in process development and manufacturing of Good Manufacturing Practice (GMP)-grade biologic and cellular therapeutic products for Phase 1/2 clinical trials. Our vision is to support the translation of ideas from academic laboratories to clinical trials.

Through Shared Resources, investigators in Fred Hutch/University of Washington Cancer Consortium and external academic and industry organizations can access services tailored to their specific research goals, including novel assay development or early access to new technologies.

Since our inception in 1995, Fred Hutch’s Antibody Technology shared resource has worked with research teams inside and outside the Hutch to design campaign strategies for the generation and identification of novel monoclonal antibody compositions. Our projects encompass a range of applications in oncology, viral therapeutics and diagnostics.

The Cellular Imaging shared resource at Fred Hutch provides advanced light microscopy services for biomedical and translational research. Our expert staff provides a complete workflow, including training for independent, 24/7 work in the core; assistance and technical support in microscopy techniques and their application; and tailored quantitative image analysis. We also offer training and support for imaging and analyzing gels and blots. We work with Fred Hutch users as well as external users from academia and industry.

Fred Hutch and Leica Microsystems have combined efforts to establish a Leica Center of Excellence in the Cellular Imaging core in support of a mission to drive new discoveries and insights from scientific research performed using the imaging systems.

The Comparative Medicine shared resource provides a variety of research support services. All our facilities are fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) International and comply with all U.S. Department of Agriculture, Public Health Service, Washington state and local area regulations.

Offers techniques to resolve structures from cellular ultrastructure to molecular interactions. Offers expertise in EM sample preparation and imaging, cryogenic electron microscopy, technologies and services, training through complexities of electron microscopy workflow, from sample preparation to image processing. All users must be trained by core staff.

Provides expertise in anatomic pathology technologies. Offers routine and special staining,  immunohistochemistry, in situ hybridization and digital pathology in support of internal and external investigators who rely on histological analysis.

Includes services from sterile cell sorting for therapeutic applications to whole cell mass spectrometry, diagnostics and early stage clinical trials. Provides expertise from experimental design to data analysis and troubleshooting, therapeutic cell sorting. Supports isolation of cells for Phase 1 clinical trials of cellular immunotherapies.

The Hutch Data Core helps investigators integrate, interpret and explore large and diverse datasets. We provide services that support efficient and reproducible analysis of large-scale datasets for biomedical researchers, drawing on deep expertise in computational biology, software engineering and cloud architecture.
We aim to make it possible for all interested investigators to apply advanced analytical techniques to complex datasets. Our diverse team offers project-based scientific analysis by expert bioinformaticians, automation of analytical pipelines using modern workflow management platforms, and publication of complex datasets with web-accelerated graphics.
In addition to direct bioinformatics support on a per-project basis, the Hutch Data Core offers training and documentation to enable scientist-driven computation. We also support automation of complex workflows for high-performance computing and provide access to web platforms specifically designed to meet researchers’ unique needs.

The Immune Monitoring shared resource supports investigators seeking precise evaluations of immunologic responses in both basic and early-stage clinical research settings. We provide users with resources and expertise to evaluate cellular immunologic processes and responses through reagents, molecular and cellular assays, assay development support and research-consulting services.

Provides in vivo imaging technology and infrastructure to support basic and preclinical research. Offers diverse array of imaging modalities, including ultrasound and optical imaging, MRI, micro-CT and multiphoton microscopy.

Provides support for mass spectrometry based proteomics and metabolomics research. With advanced instrumentation and highly trained staff, core offers consultation and services in sample preparation, sample separation, data collection and data analysis.

Supports development and maintenance of preclinical mouse models, including patient derived xenografts, cell line xenografts and genetically engineered mouse models.

The Genomics & Bioinformatics shared resource provides an integrated, streamlined and expertly supported set of services that encompass experimental design, data generation and data interpretation. Expert genomics and bioinformatics teams are available to consult and collaborate at every step of the process and work closely together to develop optimized experimental strategies to ensure that appropriate technology and data analysis tools are used to address each unique scientific question.

Provides biospecimen sample processing, extraction and biorepository services. Offers expertise to advance preclinical and clinical studies.

Core dedicated to the protocol driven collection, analysis, and reporting of behavioral data using a blend of classic and innovative assays. Supports neuroscience, psychology, pharmacology, genetics, cancer, and development by providing advanced tools and expertise for the precise measurement and interpretation of behavior.

The BRI Biorepository is responsible for the collection of samples from research participants, sample processing, storage, and sharing. We currently store samples from participants with a wide range of autoimmune diagnoses and healthy controls.

The Genomics Core provides resources to BRI and external investigators for genomics analysis, from sample processing through data generation, as well as basic bioinformatics analysis, provided by our Bioinformatics Core Team. Currently available technologies focus on Illumina RNA and DNA sequencing. RNA-seq can be performed from a variety of sample types, including purified total RNA, cell populations (including low input samples of 50-1000 cells), or single cells (either plate-sorted rare populations or high-throughput 10x single-cell analyses). DNA-seq pipelines can also be performed, including bulk CUT&Tag/CUT&RUN, and ATAC-seq and single cell ATACseq and multiome capture. Custom prepared libraries can also be submitted for sequencing.

The Core laboratory houses three Illumina sequencers, two NextSeq2000 instruments and a Miseq, plus a 10x Chromium X and a 10x CytAssist. The Chromium X is able to run GEM-X assays on fresh cells and Flex assays on fixed cells. The CytAssist works to perform spatial profiling using Visium HD technology.

Additional equipment includes a ThermoFisher Kingfisher Flex, for extracting nucleic acid from a variety of sample types using magnetic bead technology with a throughput of up to 96 samples. Two Qiagen QIAcube Connect instruments automate extraction of nucleic acid using spin column technology. Ancillary equipment for sample processing includes a Formulatrix Mantis Liquid Handler, an Agilent TapeStation 4200 (to measure RNA/DNA integrity and size), and a Tecan NanoQuant (for high throughput sample quantitation).

The Human Immunophenotyping (HIP) core at the Benaroya Research Institute (BRI) provides access to cutting-edge technologies and services enabling study of human immune-mediated diseases.

We offer expertise in human immunology along with innovative assays and state-of-the-art technology. We specialize in robust, high-parameter single-cell assays utilizing conventional, spectral, and mass cytometry (CyTOF), which can be paired with sophisticated genomic and bioninformatic analyses through our connections with other outstanding BRI cores. Other services include, but are not limited to, cell sorting, short-term in vitro assays, DNA/RNA extraction, qPCR, and ELISA or multiplex protein analysis (Luminex).

We tailor our support to meet the unique needs of each researcher. A full spectrum of services is available, from guidance on study design and training on new technologies through to execution, data analysis and interpretation. The HIP core serves investigators from BRI and Virginia Mason Medical Center (VMMC) as well as other institutions, including both non- and for-profit organizations.

The Cell and Tissue Analysis (CATA) Group at the Benaroya Research Institute offers an extensive array of flow cytometry, imaging, and histology resources for both BRI investigators as well as external research entities.

Spread over three floors at the BRI, the CATA Group strives to provide researchers with consistent and dependable access to cutting-edge technologies. With expertise in the fields of imaging, histology, and flow cytometry, we seek to not only offer the physical space and machines necessary to carry out your work but also critical support through the whole process, from hands-on training to experiment design and data analysis.