The Longitudinal Networks Core (LNC) Facility is the research arm of the Rural Drug Addiction Research (RDAR) Center and provides a suite of services, including secondary data, a participant pool for independent sampling, in-house software to collect responsive fine-grained mobile Health data from participants, support for network sampling and analytic approaches, and consultation in field-based biospecimen testing, epidemiological data collection, respondent driven sampling, prevention/intervention design, and research with hard-to-access populations.
This work was supported by the Longitudinal Networks Core Facility, the Research Core of the Rural Drug Addiction Research Center at the University of Nebraska-Lincoln. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Center or the University of Nebraska.

The UNL Nano-Engineering Research Core Facility (NERcF) is the University of Nebraska–Lincoln‘s shared research infrastructure for advanced materials characterization, micro/nanoscale manufacturing, and bioengineering research. NERcF provides open access to more than 20 state-of-the-art research instruments, technical consultation, user training, and collaborative research support for investigators from academia, government laboratories, industry, and external institutions. Major capabilities include scanning electron microscopy, focused ion beam, X-ray computed tomography, metal additive manufacturing, atomic force microscopy, nano-IR spectroscopy, optical profilometry, and mechanical characterization. By integrating advanced manufacturing with multimodal characterization, NERcF supports interdisciplinary research, workforce development, and innovation across Nebraska and beyond.

Core is dedicated process development and through GMP production facility within the UNL College of Engineering, primary focus on microbial (bacteria, yeast) expression systems. Offers biopharmaceutical process development and biomanufacturing services that transition discoveries to non-clinical studies and Phase I/II and early Phase III clinical trials.

Core provides instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. The services that our core provides can be extended to partially purified proteins, whole cell assays and analysis of tissues. Equipment includes triple-quadrupole LC-MS/MS, ICP-MS for elemental analysis, circular dichroism, biolayer interference, stopped flow, Isothermal calorimeter, differential scanning calorimeter, steady-state fluorescence and polarization of fluorescence.

Facility provides access to Chromium iX platform from 10X Genomics. This allows user to barcode single cells or nuclei and process them for single cell RNA-seq, single nucleus ATAC-seq or single nucleus Multiome ATC + Gene Expression protocols.

Provides range of evaluation and data services to support client needs.Through consulting, evaluation, methodology, and training, we work to fill gaps and improve and empower data driven decision making.At the proposal stage of grants, we can design evaluation plans to meet your project needs, with the associated narrative text, budget, and letter of collaboration, as needed. We can also fill gaps post-award, or provide support with internal funding. We can assist with logic models, Broader Impacts, methodologies, needs assessments, survey design and implementation, support with Institutional Review Board applications, and trainings in our areas of expertise.

The Proteomics and Metabolomics Facility (PMF) at the Center for Biotechnology was established in 2015 under the leadership of the Director, Dr. Sophie Alvarez and the Assistant Director, Dr. Mike Naldrett to meet ever increasing needs for a range of technical services using mass spectrometry, including identification and relative quantification of proteins and several advanced methods for profiling and quantitation of small molecules.

The primary aim of the PMF is to provide high quality service and collaborative work in the fields of Proteomics and Metabolomics to support scientists and students within our University community and around the world. Through this we strive to advance world-class research, support grant proposals, assist in the education of future scientists and support economic and scientific advancements. We offer both Full-Service and Self-Service options to meet the unique needs, time requirements and budgets of our users. Our outreach and education program includes workshops, seminars and courses.

Proteomics services include:

Bottom-up Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels.
Quantification by label-free or multiplexed labeling techniques (eg TMT-10/16plex) with microscale subfractionation to increase coverage.
Post Translational Modification Characterization especially phosphopeptide enrichment from whole organism digests, but also ubiquitylation, acetylation, etc.

Metabolomics services include:

Untargeted Quantification of a wide range of small molecules
Primary metabolism using GC-MS and NIST/Fiehn Libraries
Secondary metabolism using LC-MS and Progenesis QI, NIST Library, mzCloud
Targeted Quantification using HPLC or LCMS.

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The Flow Cytometry Core Facility provides University of Nebraska faculty, staff, students, and external academic and industry with high quality flow cytometry services.

The facility currently has a Beckman Coulter CytoFLEX SRT sorter and CytoFLEX LX, as well as a NanoAnalyzer from NanoFCM.

Facility staff are able to assist with everything from experimental design, instrument training, sample collection, and data analysis.

The CryoEM core facility is dedicated to the structural characterization of biological samples and microcrystals using cryo-electron microscopy (CryoEM). The core can run the main three CryoEM workflows, including, single-particle CryoEM, Cryo-electron tomography (CryoET), and Micro crystal electron diffraction (MicroED). We have state-of-the-art instrumentation that includes a TFS 200kV Glacios CryoTEM, equipped with a zero-loss Selectris Energy Filter, a Flacon 4i Direct Electron Detector, and a Ceta-D camera. In addition, the facility counts with a Pelco Easy Glow Discharger and two vitrification robots, the TFS Vitrobot Mark IV, and the Leica EM GP2. We also present all the capacities for storing and shipping samples. Furthermore, our facility has a dedicated Data storage Server and a Live processing workstation for our users‘ convenience, including the data processing suites RELION and CryoSPARC available at our campus supercomputer Swan cluster at the Holland Computer Center.

The UNL Bioinformatics Core Research Facility (BCRF_ helps researchers, both in academic institutions and for-profits, with the analysis of 'omics data and the integration thereof. We offer, inter alia, gene expression analysis, single-cell/nuclei RNA-seq analysis, transcriptome and genome assembly with gene prediction and annotation, and statistical analysis.

Morrison Microscopy Core Research Facility
Located on the first floor of the George W. Beadle building, the Morrison Microscopy Core Research Facility has state of the art imaging systems including digital microscopes, confocal laser scanning microscope and three electron microscopes. The microscopy core facility will provide strong technical support for multi-disciplinary research in the state of Nebraska. We will also provide services, teaching and training for university research communities.